Show simple item record

dc.contributor.authorMok, PL
dc.contributor.authorLeow, SN
dc.contributor.authorKoh, AE-H
dc.contributor.authorNizam, HHM
dc.contributor.authorDing, SLS
dc.contributor.authorLuu, C
dc.contributor.authorRuhaslizan, R
dc.contributor.authorWong, HS
dc.contributor.authorHalim, WHWA
dc.contributor.authorNg, MH
dc.contributor.authorIdrus, RBH
dc.contributor.authorChowdhury, SR
dc.contributor.authorBastion, CM-L
dc.contributor.authorSubbiah, SK
dc.contributor.authorHiguchi, A
dc.contributor.authorAlarfaj, AA
dc.contributor.authorThen, KY
dc.date.accessioned2020-12-22T04:08:50Z
dc.date.available2020-12-22T04:08:50Z
dc.date.issued2017-02-01
dc.identifierpii: ijms18020345
dc.identifier.citationMok, P. L., Leow, S. N., Koh, A. E. -H., Nizam, H. H. M., Ding, S. L. S., Luu, C., Ruhaslizan, R., Wong, H. S., Halim, W. H. W. A., Ng, M. H., Idrus, R. B. H., Chowdhury, S. R., Bastion, C. M. -L., Subbiah, S. K., Higuchi, A., Alarfaj, A. A. & Then, K. Y. (2017). Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18 (2), https://doi.org/10.3390/ijms18020345.
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/11343/258005
dc.description.abstractMesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.
dc.languageEnglish
dc.publisherMDPI
dc.titleMicro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
dc.typeJournal Article
dc.identifier.doi10.3390/ijms18020345
melbourne.affiliation.departmentOphthalmology (Eye & Ear Hospital)
melbourne.affiliation.departmentCentre for Eye Research Australia (CERA)
melbourne.source.titleInternational Journal of Molecular Sciences
melbourne.source.volume18
melbourne.source.issue2
dc.rights.licenseCC BY
melbourne.elementsid1183691
melbourne.contributor.authorLuu, Chi
melbourne.contributor.authorLuu, Chi
dc.identifier.eissn1422-0067
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record