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dc.contributor.authorLum, M
dc.contributor.authorTurbic, A
dc.contributor.authorMitrovic, B
dc.contributor.authorTurnley, AM
dc.date.available2014-05-21T19:08:32Z
dc.date.issued2009-08-01
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000267450700002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=d4d813f4571fa7d6246bdc0dfeca3a1c
dc.identifier.citationLum, M., Turbic, A., Mitrovic, B. & Turnley, A. M. (2009). Fibroblast Growth Factor-9 Inhibits Astrocyte Differentiation of Adult Mouse Neural Progenitor Cells. JOURNAL OF NEUROSCIENCE RESEARCH, 87 (10), pp.2201-2210. https://doi.org/10.1002/jnr.22047.
dc.identifier.issn0360-4012
dc.identifier.urihttp://hdl.handle.net/11343/25802
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractFibroblast growth factor-9 (FGF9) is expressed in the CNS and is reported to be a mitogen for glial cells, to promote neuronal survival, and to retard oligodendrocyte differentiation. Here we examined the effects of FGF9 on the differentiation, survival, and proliferation of adult neural progenitor cells derived from the adult mouse subventricular zone. FGF9 by itself induced neurosphere proliferation, but its effects were modest compared with those of epidermal growth factor and FGF2. When neurospheres were dissociated and plated for differentiation, FGF9 increased total cell number over time in a dose-dependent manner. Ki67 immunostaining and bromodeoxyuridine incorporation indicated that this was at least partially due to the continued presence of proliferative nestin-positive neural progenitor cells and betaIII tubulin-positive neuronal precursors. FGF9 also promoted cell survival as indicated by a decreased number of TUNEL-positive cells over time. Assessment of differentiation showed that FGF9 increased neuron generation that reflected the increase in total cell number; however, the percentage of progenitor cells differentiating into neurons was slightly decreased. FGF9 had a modest effect on oligodendrocyte generation, although it appeared to slow the maturation of oligodenrocytes at higher concentrations. The most marked effect on differentiation was an almost total lack of glial fibrillary acidic protein (GFAP)-positive astrocytes up to 7 days following FGF9 addition, indicating that astrocyte differentiation was strongly inhibited. Total inhibition required prolonged treatment, although a 1-hr pulse was sufficient for partial inhibition, and bone morphogenic protein-4 could partially overcome the FGF9 inhibition of astrocyte differentiation. FGF9 therefore has multiple effects on adult neural precursor cell function, enhancing neuronal precursor proliferation and specifically inhibiting GFAP expression.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherWILEY-BLACKWELL
dc.relation.isversionofhttp://tinyurl.com/p8ddt5
dc.subjectCentral Nervous System; Nervous System and Disorders
dc.titleFibroblast Growth Factor-9 Inhibits Astrocyte Differentiation of Adult Mouse Neural Progenitor Cells
dc.typeJournal Article
dc.identifier.doi10.1002/jnr.22047
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMedicine, Dentistry And Health Sciences
melbourne.source.titleJOURNAL OF NEUROSCIENCE RESEARCH
melbourne.source.volume87
melbourne.source.issue10
melbourne.source.pages2201-2210
dc.research.codefor110903
dc.research.coderfcd320702
dc.research.codeseo1998730104
dc.research.codeseo2008920111
melbourne.publicationid121111
melbourne.elementsid308950
melbourne.contributor.authorTurbic, Alisa
melbourne.contributor.authorTurnley, Ann
melbourne.contributor.authorLUM, MAY
dc.identifier.eissn1097-4547
melbourne.accessrightsThis item is currently not available from this repository


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