Show simple item record

dc.contributor.authorHuntington, ND
dc.contributor.authorXu, YK
dc.contributor.authorNutt, SL
dc.contributor.authorTarlinton, DM
dc.date.accessioned2020-12-22T04:18:26Z
dc.date.available2020-12-22T04:18:26Z
dc.date.issued2005-05-02
dc.identifierpii: jem.20042294
dc.identifier.citationHuntington, N. D., Xu, Y. K., Nutt, S. L. & Tarlinton, D. M. (2005). A requirement for CD45 distinguishes Ly49D-mediated cytokine and chemokine production from killing in primary natural killer cells. JOURNAL OF EXPERIMENTAL MEDICINE, 201 (9), pp.1421-1433. https://doi.org/10.1084/jem.20042294.
dc.identifier.issn0022-1007
dc.identifier.urihttp://hdl.handle.net/11343/258039
dc.description.abstractEngagement of receptors on the surface of natural killer (NK) cells initiates a biochemical cascade ultimately triggering cytokine production and cytotoxicity, although the interrelationship between these two outcomes is currently unclear. In this study we investigate the role of the cell surface phosphatase CD45 in NK cell development and intracellular signaling from activating receptors. Stimulation via the major histocompatibility complex I-binding receptor, Ly49D on CD45(-/-) primary NK cells resulted in the activation of phosphoinositide-3-kinase and normal cytotoxicity but failed to elicit a range of cytokines and chemokines. This blockage is associated with impaired phosphorylation of Syk, Vav1, JNK, and p38, which mimics data obtained using inhibitors of the src-family kinases (SFK). These data, supported by analogous findings after CD16 and NKG2D stimulation of CD45(-/-) primary NK cells, place CD45 upstream of SFK in NK cells after stimulation via immunoreceptor tyrosine-based activation motif-containing receptors. Thus we identify CD45 as a pivotal enzyme in eliciting a precise subset of NK cell responses.
dc.languageEnglish
dc.publisherROCKEFELLER UNIV PRESS
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.titleA requirement for CD45 distinguishes Ly49D-mediated cytokine and chemokine production from killing in primary natural killer cells
dc.typeJournal Article
dc.identifier.doi10.1084/jem.20042294
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleJournal of Experimental Medicine
melbourne.source.volume201
melbourne.source.issue9
melbourne.source.pages1421-1433
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1022944
melbourne.contributor.authorNutt, Stephen
melbourne.contributor.authorHuntington, Nicholas
dc.identifier.eissn1540-9538
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record