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dc.contributor.authorSTEWART, AG
dc.contributor.authorDUSTING, GJ
dc.contributor.authorGIARRACCA, RG
dc.contributor.authorHARRIS, T
dc.contributor.authorLIM, Y
dc.contributor.authorSOBEY, CG
dc.date.accessioned2020-12-22T04:28:09Z
dc.date.available2020-12-22T04:28:09Z
dc.date.issued1993-10-01
dc.identifier.citationSTEWART, A. G., DUSTING, G. J., GIARRACCA, R. G., HARRIS, T., LIM, Y. & SOBEY, C. G. (1993). NITRITE IS PRODUCED BY ELICITED BUT NOT BY CIRCULATING NEUTROPHILS. MEDIATORS OF INFLAMMATION, 2 (5), pp.349-356. https://doi.org/10.1155/S0962935193000481.
dc.identifier.issn0962-9351
dc.identifier.urihttp://hdl.handle.net/11343/258075
dc.description.abstractThe generation of nitrite (NO(2) (-)) was used as an index of the production of nitric oxide by human and rat polymorphonuclear leukocytes (PMN) and rat peritoneal macrophages. Human peripheral blood PMN did not produce significant levels of NO(2) (-). Attempts to induce NO(2) (-) generation in human PMN by incubation with GM-CSF (1 nM), TNFalpha (0.3 nM), endotoxin (1 mug/ml) or formyl-Met-Leu-Phe (100 nM) for up to 16 h were not successful. Addition of human PMN primed by GM-CSF (1 nM) to rabbit aortic ring preparations precontracted with phenylephrine had no effect on tone. In contrast to these observations, PMN, isolated from the peritoneum of oyster glycogen treated rats, generated NO(2) (-) via a pathway sensitive to inhibition by the nitric oxide synthase inhibitor, N(G)-monomethyl L-arginine. However, peripheral blood rat PMN obtained from the same animals did not produce NO(2) (-), even during prolonged incubation for periods of up to 16 h. It is suggested that detectable NO production by PMN requires NO synthase activity to be induced either by the process of PMN migration or by exposure to certain cytokines produced locally at the site of inflammation.
dc.languageEnglish
dc.publisherRAPID SCIENCE PUBLISHERS
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleNITRITE IS PRODUCED BY ELICITED BUT NOT BY CIRCULATING NEUTROPHILS
dc.typeJournal Article
dc.identifier.doi10.1155/S0962935193000481
melbourne.affiliation.departmentPharmacology and Therapeutics
melbourne.affiliation.departmentSurgery (St Vincent's)
melbourne.source.titleMediators of Inflammation
melbourne.source.volume2
melbourne.source.issue5
melbourne.source.pages349-356
dc.rights.licenseCC BY
melbourne.elementsid1028296
melbourne.contributor.authorStewart, Alastair
melbourne.contributor.authorDusting, Gregory
dc.identifier.eissn1466-1861
melbourne.accessrightsOpen Access


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