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dc.contributor.authorGillespie, L
dc.contributor.authorRoosendahl, P
dc.contributor.authorNg, WC
dc.contributor.authorBrooks, AG
dc.contributor.authorReading, PC
dc.contributor.authorLondrigan, SL
dc.date.accessioned2020-12-22T04:31:55Z
dc.date.available2020-12-22T04:31:55Z
dc.date.issued2016-01-14
dc.identifierpii: srep19428
dc.identifier.citationGillespie, L., Roosendahl, P., Ng, W. C., Brooks, A. G., Reading, P. C. & Londrigan, S. L. (2016). Endocytic function is critical for influenza A virus infection via DC-SIGN and L-SIGN. SCIENTIFIC REPORTS, 6 (1), https://doi.org/10.1038/srep19428.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/258085
dc.description.abstractThe ubiquitous presence of cell-surface sialic acid (SIA) has complicated efforts to identify specific transmembrane glycoproteins that function as bone fide entry receptors for influenza A virus (IAV) infection. The C-type lectin receptors (CLRs) DC-SIGN (CD209) and L-SIGN (CD209L) enhance IAV infection however it is not known if they act as attachment factors, passing virions to other unknown receptors for virus entry, or as authentic entry receptors for CLR-mediated virus uptake and infection. Sialic acid-deficient Lec2 Chinese Hamster Ovary (CHO) cell lines were resistant to IAV infection whereas expression of DC-SIGN/L-SIGN restored susceptibility of Lec2 cells to pH- and dynamin-dependent infection. Moreover, Lec2 cells expressing endocytosis-defective DC-SIGN/L-SIGN retained capacity to bind IAV but showed reduced susceptibility to infection. These studies confirm that DC-SIGN and L-SIGN are authentic endocytic receptors for IAV entry and infection.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleEndocytic function is critical for influenza A virus infection via DC-SIGN and L-SIGN
dc.typeJournal Article
dc.identifier.doi10.1038/srep19428
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.departmentDoherty Institute
melbourne.source.titleScientific Reports
melbourne.source.volume6
melbourne.source.issue1
melbourne.identifier.nhmrc1027545
dc.rights.licenseCC BY
melbourne.elementsid1029780
melbourne.contributor.authorReading, Patrick
melbourne.contributor.authorLondrigan, Sarah
melbourne.contributor.authorBrooks, Andrew
melbourne.contributor.authorGillespie, Leah
melbourne.contributor.authorROOSENDAHL, PAULA
melbourne.contributor.authorNg, Wei Yi
dc.identifier.eissn2045-2322
melbourne.identifier.fundernameidNHMRC, 1027545
melbourne.accessrightsOpen Access


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