| dc.identifier.citation | Trijatmiko, K. R., Duenas, C., Tsakirpaloglou, N., Torrizo, L., Arines, F. M., Adeva, C., Balindong, J., Oliva, N., Sapasap, M. V., Borrero, J., Rey, J., Francisco, P., Nelson, A., Nakanishi, H., Lombi, E., Tako, E., Glahn, R. P., Stangoulis, J., Chadha-Mohanty, P. ,... Slamet-Loedin, I. H. (2016). Biofortified indica rice attains iron and zinc nutrition dietary targets in the field. SCIENTIFIC REPORTS, 6 (1), https://doi.org/10.1038/srep19792. | |
| dc.description.abstract | More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g(-1) iron (Fe) and 16 μg g(-1) zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g(-1) Fe and 28 μg g(-1) Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g(-1) in glasshouse settings; in contrast, under field conditions, 4 μg g(-1) was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g(-1) Fe and 45.7 μg g(-1) Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds. | |
