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    The role of the Hes1 crosstalk hub in Notch-Wnt interactions of the intestinal crypt

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    17
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    Author
    Kay, SK; Harrington, HA; Shepherd, S; Brennan, K; Dale, T; Osborne, JM; Gavaghan, DJ; Byrne, HM
    Date
    2017-02-01
    Source Title
    PLoS Computational Biology
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Osborne, James
    Affiliation
    School of Mathematics and Statistics
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Kay, S. K., Harrington, H. A., Shepherd, S., Brennan, K., Dale, T., Osborne, J. M., Gavaghan, D. J. & Byrne, H. M. (2017). The role of the Hes1 crosstalk hub in Notch-Wnt interactions of the intestinal crypt. PLOS COMPUTATIONAL BIOLOGY, 13 (2), https://doi.org/10.1371/journal.pcbi.1005400.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/258194
    DOI
    10.1371/journal.pcbi.1005400
    Abstract
    The Notch pathway plays a vital role in determining whether cells in the intestinal epithelium adopt a secretory or an absorptive phenotype. Cell fate specification is coordinated via Notch's interaction with the canonical Wnt pathway. Here, we propose a new mathematical model of the Notch and Wnt pathways, in which the Hes1 promoter acts as a hub for pathway crosstalk. Computational simulations of the model can assist in understanding how healthy intestinal tissue is maintained, and predict the likely consequences of biochemical knockouts upon cell fate selection processes. Chemical reaction network theory (CRNT) is a powerful, generalised framework which assesses the capacity of our model for monostability or multistability, by analysing properties of the underlying network structure without recourse to specific parameter values or functional forms for reaction rates. CRNT highlights the role of β-catenin in stabilising the Notch pathway and damping oscillations, demonstrating that Wnt-mediated actions on the Hes1 promoter can induce dynamic transitions in the Notch system, from multistability to monostability. Time-dependent model simulations of cell pairs reveal the stabilising influence of Wnt upon the Notch pathway, in which β-catenin- and Dsh-mediated action on the Hes1 promoter are key in shaping the subcellular dynamics. Where Notch-mediated transcription of Hes1 dominates, there is Notch oscillation and maintenance of fate flexibility; Wnt-mediated transcription of Hes1 favours bistability akin to cell fate selection. Cells could therefore regulate the proportion of Wnt- and Notch-mediated control of the Hes1 promoter to coordinate the timing of cell fate selection as they migrate through the intestinal epithelium and are subject to reduced Wnt stimuli. Furthermore, mutant cells characterised by hyperstimulation of the Wnt pathway may, through coupling with Notch, invert cell fate in neighbouring healthy cells, enabling an aberrant cell to maintain its neighbours in mitotically active states.

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