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    Non-Invasive In Vivo Imaging of Calcium Signaling in Mice

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    Author
    Rogers, KL; Picaud, S; Roncali, E; Boisgard, R; Colasante, C; Stinnakre, J; Tavitian, B; Brulet, P
    Date
    2007-10-03
    Source Title
    PLoS One
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Rogers, Kelly
    Affiliation
    Medical Biology (W.E.H.I.)
    Metadata
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    Document Type
    Journal Article
    Citations
    Rogers, K. L., Picaud, S., Roncali, E., Boisgard, R., Colasante, C., Stinnakre, J., Tavitian, B. & Brulet, P. (2007). Non-Invasive In Vivo Imaging of Calcium Signaling in Mice. PLOS ONE, 2 (10), https://doi.org/10.1371/journal.pone.0000974.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/258231
    DOI
    10.1371/journal.pone.0000974
    Abstract
    Rapid and transient elevations of Ca(2+) within cellular microdomains play a critical role in the regulation of many signal transduction pathways. Described here is a genetic approach for non-invasive detection of localized Ca(2+) concentration ([Ca(2+)]) rises in live animals using bioluminescence imaging (BLI). Transgenic mice conditionally expressing the Ca(2+)-sensitive bioluminescent reporter GFP-aequorin targeted to the mitochondrial matrix were studied in several experimental paradigms. Rapid [Ca(2+)] rises inside the mitochondrial matrix could be readily detected during single-twitch muscle contractions. Whole body patterns of [Ca(2+)] were monitored in freely moving mice and during epileptic seizures. Furthermore, variations in mitochondrial [Ca(2+)] correlated to behavioral components of the sleep/wake cycle were observed during prolonged whole body recordings of newborn mice. This non-invasive imaging technique opens new avenues for the analysis of Ca(2+) signaling whenever whole body information in freely moving animals is desired, in particular during behavioral and developmental studies.

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