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dc.contributor.authorRogers, KL
dc.contributor.authorPicaud, S
dc.contributor.authorRoncali, E
dc.contributor.authorBoisgard, R
dc.contributor.authorColasante, C
dc.contributor.authorStinnakre, J
dc.contributor.authorTavitian, B
dc.contributor.authorBrulet, P
dc.date.accessioned2020-12-22T05:11:29Z
dc.date.available2020-12-22T05:11:29Z
dc.date.issued2007-10-03
dc.identifier.citationRogers, K. L., Picaud, S., Roncali, E., Boisgard, R., Colasante, C., Stinnakre, J., Tavitian, B. & Brulet, P. (2007). Non-Invasive In Vivo Imaging of Calcium Signaling in Mice. PLOS ONE, 2 (10), https://doi.org/10.1371/journal.pone.0000974.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/258231
dc.description.abstractRapid and transient elevations of Ca(2+) within cellular microdomains play a critical role in the regulation of many signal transduction pathways. Described here is a genetic approach for non-invasive detection of localized Ca(2+) concentration ([Ca(2+)]) rises in live animals using bioluminescence imaging (BLI). Transgenic mice conditionally expressing the Ca(2+)-sensitive bioluminescent reporter GFP-aequorin targeted to the mitochondrial matrix were studied in several experimental paradigms. Rapid [Ca(2+)] rises inside the mitochondrial matrix could be readily detected during single-twitch muscle contractions. Whole body patterns of [Ca(2+)] were monitored in freely moving mice and during epileptic seizures. Furthermore, variations in mitochondrial [Ca(2+)] correlated to behavioral components of the sleep/wake cycle were observed during prolonged whole body recordings of newborn mice. This non-invasive imaging technique opens new avenues for the analysis of Ca(2+) signaling whenever whole body information in freely moving animals is desired, in particular during behavioral and developmental studies.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleNon-Invasive In Vivo Imaging of Calcium Signaling in Mice
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0000974
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titlePLoS One
melbourne.source.volume2
melbourne.source.issue10
dc.rights.licenseCC BY
melbourne.elementsid1190214
melbourne.contributor.authorRogers, Kelly
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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