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dc.contributor.authorLee, D-S
dc.contributor.authorShin, J-Y
dc.contributor.authorTonge, PD
dc.contributor.authorPuri, MC
dc.contributor.authorLee, S
dc.contributor.authorPark, H
dc.contributor.authorLee, W-C
dc.contributor.authorHussein, SMI
dc.contributor.authorBleazard, T
dc.contributor.authorYun, J-Y
dc.contributor.authorKim, J
dc.contributor.authorLi, M
dc.contributor.authorCloonan, N
dc.contributor.authorWood, D
dc.contributor.authorClancy, JL
dc.contributor.authorMosbergen, R
dc.contributor.authorYi, J-H
dc.contributor.authorYang, K-S
dc.contributor.authorKim, H
dc.contributor.authorRhee, H
dc.contributor.authorWells, CA
dc.contributor.authorPreiss, T
dc.contributor.authorGrimmond, SM
dc.contributor.authorRogers, IM
dc.contributor.authorNagy, A
dc.contributor.authorSeo, J-S
dc.date.accessioned2020-12-22T05:13:15Z
dc.date.available2020-12-22T05:13:15Z
dc.date.issued2014-12-01
dc.identifierpii: ncomms6619
dc.identifier.citationLee, D. -S., Shin, J. -Y., Tonge, P. D., Puri, M. C., Lee, S., Park, H., Lee, W. -C., Hussein, S. M. I., Bleazard, T., Yun, J. -Y., Kim, J., Li, M., Cloonan, N., Wood, D., Clancy, J. L., Mosbergen, R., Yi, J. -H., Yang, K. -S., Kim, H. ,... Seo, J. -S. (2014). An epigenomic roadmap to induced pluripotency reveals DNA methylation as a reprogramming modulator. NATURE COMMUNICATIONS, 5 (1), https://doi.org/10.1038/ncomms6619.
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/11343/258237
dc.description.abstractReprogramming of somatic cells to induced pluripotent stem cells involves a dynamic rearrangement of the epigenetic landscape. To characterize this epigenomic roadmap, we have performed MethylC-seq, ChIP-seq (H3K4/K27/K36me3) and RNA-Seq on samples taken at several time points during murine secondary reprogramming as part of Project Grandiose. We find that DNA methylation gain during reprogramming occurs gradually, while loss is achieved only at the ESC-like state. Binding sites of activated factors exhibit focal demethylation during reprogramming, while ESC-like pluripotent cells are distinguished by extension of demethylation to the wider neighbourhood. We observed that genes with CpG-rich promoters demonstrate stable low methylation and strong engagement of histone marks, whereas genes with CpG-poor promoters are safeguarded by methylation. Such DNA methylation-driven control is the key to the regulation of ESC-pluripotency genes, including Dppa4, Dppa5a and Esrrb. These results reveal the crucial role that DNA methylation plays as an epigenetic switch driving somatic cells to pluripotency.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.titleAn epigenomic roadmap to induced pluripotency reveals DNA methylation as a reprogramming modulator
dc.typeJournal Article
dc.identifier.doi10.1038/ncomms6619
melbourne.affiliation.departmentCentre for Cancer Research
melbourne.affiliation.departmentSchool of Historical and Philosophical Studies
melbourne.affiliation.departmentAnatomy and Neuroscience
melbourne.source.titleNature Communications
melbourne.source.volume5
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1033252
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284806
melbourne.contributor.authorGrimmond, Sean
melbourne.contributor.authorMosbergen, Rowland
melbourne.contributor.authorWells, Christine
dc.identifier.eissn2041-1723
melbourne.accessrightsOpen Access


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