University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Medical Biology
  • Medical Biology - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Medical Biology
  • Medical Biology - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

    Thumbnail
    Download
    Published version (205.6Kb)

    Citations
    Scopus
    Web of Science
    Altmetric
    2
    2
    Author
    Dragavon, J; Sinow, C; Holland, AD; Rekiki, A; Theodorou, I; Samson, C; Blazquez, S; Rogers, KL; Tournebize, R; Shorte, SL
    Date
    2014-05-01
    Source Title
    Journal of Visualized Experiments
    Publisher
    JOURNAL OF VISUALIZED EXPERIMENTS
    University of Melbourne Author/s
    Rogers, Kelly
    Affiliation
    Medical Biology (W.E.H.I.)
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Dragavon, J., Sinow, C., Holland, A. D., Rekiki, A., Theodorou, I., Samson, C., Blazquez, S., Rogers, K. L., Tournebize, R. & Shorte, S. L. (2014). A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL). JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (87), https://doi.org/10.3791/51549.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/258263
    DOI
    10.3791/51549
    Abstract
    Fluorescence by Unbound Excitation from Luminescence (FUEL) is a radiative excitation-emission process that produces increased signal and contrast enhancement in vitro and in vivo. FUEL shares many of the same underlying principles as Bioluminescence Resonance Energy Transfer (BRET), yet greatly differs in the acceptable working distances between the luminescent source and the fluorescent entity. While BRET is effectively limited to a maximum of 2 times the Förster radius, commonly less than 14 nm, FUEL can occur at distances up to µm or even cm in the absence of an optical absorber. Here we expand upon the foundation and applicability of FUEL by reviewing the relevant principles behind the phenomenon and demonstrate its compatibility with a wide variety of fluorophores and fluorescent nanoparticles. Further, the utility of antibody-targeted FUEL is explored. The examples shown here provide evidence that FUEL can be utilized for applications where BRET is not possible, filling the spatial void that exists between BRET and traditional whole animal imaging.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52443]
    • Medical Biology - Research Publications [1412]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors