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dc.contributor.authorDragavon, J
dc.contributor.authorSinow, C
dc.contributor.authorHolland, AD
dc.contributor.authorRekiki, A
dc.contributor.authorTheodorou, I
dc.contributor.authorSamson, C
dc.contributor.authorBlazquez, S
dc.contributor.authorRogers, KL
dc.contributor.authorTournebize, R
dc.contributor.authorShorte, SL
dc.date.accessioned2020-12-22T05:20:00Z
dc.date.available2020-12-22T05:20:00Z
dc.date.issued2014-05-01
dc.identifier.citationDragavon, J., Sinow, C., Holland, A. D., Rekiki, A., Theodorou, I., Samson, C., Blazquez, S., Rogers, K. L., Tournebize, R. & Shorte, S. L. (2014). A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL). JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (87), https://doi.org/10.3791/51549.
dc.identifier.issn1940-087X
dc.identifier.urihttp://hdl.handle.net/11343/258263
dc.description.abstractFluorescence by Unbound Excitation from Luminescence (FUEL) is a radiative excitation-emission process that produces increased signal and contrast enhancement in vitro and in vivo. FUEL shares many of the same underlying principles as Bioluminescence Resonance Energy Transfer (BRET), yet greatly differs in the acceptable working distances between the luminescent source and the fluorescent entity. While BRET is effectively limited to a maximum of 2 times the Förster radius, commonly less than 14 nm, FUEL can occur at distances up to µm or even cm in the absence of an optical absorber. Here we expand upon the foundation and applicability of FUEL by reviewing the relevant principles behind the phenomenon and demonstrate its compatibility with a wide variety of fluorophores and fluorescent nanoparticles. Further, the utility of antibody-targeted FUEL is explored. The examples shown here provide evidence that FUEL can be utilized for applications where BRET is not possible, filling the spatial void that exists between BRET and traditional whole animal imaging.
dc.languageEnglish
dc.publisherJOURNAL OF VISUALIZED EXPERIMENTS
dc.titleA Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
dc.typeJournal Article
dc.identifier.doi10.3791/51549
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleJournal of Visualized Experiments
melbourne.source.issue87
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1190207
melbourne.contributor.authorRogers, Kelly
dc.identifier.eissn1940-087X
melbourne.accessrightsOpen Access


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