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    Reference datasets of tufAle and UPA markers to identify algae in metabarcoding surveys

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    Author
    Marcelino, VR; Verbruggen, H
    Date
    2017-04-01
    Source Title
    Data in Brief
    Publisher
    ELSEVIER SCIENCE BV
    University of Melbourne Author/s
    Verbruggen, Heroen
    Affiliation
    School of BioSciences
    Metadata
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    Document Type
    Journal Article
    Citations
    Marcelino, V. R. & Verbruggen, H. (2017). Reference datasets of tufAle and UPA markers to identify algae in metabarcoding surveys. DATA IN BRIEF, 11, pp.273-276. https://doi.org/10.1016/j.dib.2017.02.013.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/258290
    DOI
    10.1016/j.dib.2017.02.013
    Abstract
    The data presented here are related to the research article "Multi-marker metabarcoding of coral skeletons reveals a rich microbiome and diverse evolutionary origins of endolithic algae" (Marcelino and Verbruggen, 2016) [1]. Here we provide reference datasets of the elongation factor Tu (tufA) and the Universal Plastid Amplicon (UPA) markers in a format that is ready-to-use in the QIIME pipeline (Caporaso et al., 2010) [2]. In addition to sequences previously available in GenBank, we included newly discovered endolithic algae lineages using both amplicon sequencing (Marcelino and Verbruggen, 2016) [1] and chloroplast genome data (Marcelino et al., 2016; Verbruggen et al., in press) [3], [4]. We also provide a script to convert GenBank flatfiles into reference datasets that can be used with other markers. The tufA and UPA reference datasets are made publicly available here to facilitate biodiversity assessments of microalgal communities.

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