Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

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Trussart, M; Yus, E; Martinez, S; Bau, D; Tahara, YO; Pengo, T; Widjaja, M; Kretschmer, S; Swoger, J; Djordjevic, S; ...Date
2017-03-08Source Title
Nature CommunicationsPublisher
NATURE PUBLISHING GROUPUniversity of Melbourne Author/s
Trussart, MarieAffiliation
Medical Biology (W.E.H.I.)Metadata
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Journal ArticleCitations
Trussart, M., Yus, E., Martinez, S., Bau, D., Tahara, Y. O., Pengo, T., Widjaja, M., Kretschmer, S., Swoger, J., Djordjevic, S., Turnbull, L., Whitchurch, C., Miyata, M., Marti-Renom, M. A., Lluch-Senar, M. & Serrano, L. (2017). Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae. NATURE COMMUNICATIONS, 8 (1), https://doi.org/10.1038/ncomms14665.Access Status
Open AccessAbstract
DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.
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