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    Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

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    Author
    Trussart, M; Yus, E; Martinez, S; Bau, D; Tahara, YO; Pengo, T; Widjaja, M; Kretschmer, S; Swoger, J; Djordjevic, S; ...
    Date
    2017-03-08
    Source Title
    Nature Communications
    Publisher
    NATURE PUBLISHING GROUP
    University of Melbourne Author/s
    Trussart, Marie
    Affiliation
    Medical Biology (W.E.H.I.)
    Metadata
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    Document Type
    Journal Article
    Citations
    Trussart, M., Yus, E., Martinez, S., Bau, D., Tahara, Y. O., Pengo, T., Widjaja, M., Kretschmer, S., Swoger, J., Djordjevic, S., Turnbull, L., Whitchurch, C., Miyata, M., Marti-Renom, M. A., Lluch-Senar, M. & Serrano, L. (2017). Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae. NATURE COMMUNICATIONS, 8 (1), https://doi.org/10.1038/ncomms14665.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/258300
    DOI
    10.1038/ncomms14665
    Abstract
    DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.

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