Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae
dc.contributor.author | Trussart, M | |
dc.contributor.author | Yus, E | |
dc.contributor.author | Martinez, S | |
dc.contributor.author | Bau, D | |
dc.contributor.author | Tahara, YO | |
dc.contributor.author | Pengo, T | |
dc.contributor.author | Widjaja, M | |
dc.contributor.author | Kretschmer, S | |
dc.contributor.author | Swoger, J | |
dc.contributor.author | Djordjevic, S | |
dc.contributor.author | Turnbull, L | |
dc.contributor.author | Whitchurch, C | |
dc.contributor.author | Miyata, M | |
dc.contributor.author | Marti-Renom, MA | |
dc.contributor.author | Lluch-Senar, M | |
dc.contributor.author | Serrano, L | |
dc.date.accessioned | 2020-12-22T05:29:24Z | |
dc.date.available | 2020-12-22T05:29:24Z | |
dc.date.issued | 2017-03-08 | |
dc.identifier | pii: ncomms14665 | |
dc.identifier.citation | Trussart, M., Yus, E., Martinez, S., Bau, D., Tahara, Y. O., Pengo, T., Widjaja, M., Kretschmer, S., Swoger, J., Djordjevic, S., Turnbull, L., Whitchurch, C., Miyata, M., Marti-Renom, M. A., Lluch-Senar, M. & Serrano, L. (2017). Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae. NATURE COMMUNICATIONS, 8 (1), https://doi.org/10.1038/ncomms14665. | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | http://hdl.handle.net/11343/258300 | |
dc.description.abstract | DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems. | |
dc.language | English | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.title | Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae | |
dc.type | Journal Article | |
dc.identifier.doi | 10.1038/ncomms14665 | |
melbourne.affiliation.department | Medical Biology (W.E.H.I.) | |
melbourne.source.title | Nature Communications | |
melbourne.source.volume | 8 | |
melbourne.source.issue | 1 | |
dc.rights.license | CC BY | |
melbourne.elementsid | 1190699 | |
melbourne.contributor.author | Trussart, Marie | |
dc.identifier.eissn | 2041-1723 | |
melbourne.accessrights | Open Access |
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Medical Biology - Research Publications [1412]