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dc.contributor.authorTrussart, M
dc.contributor.authorYus, E
dc.contributor.authorMartinez, S
dc.contributor.authorBau, D
dc.contributor.authorTahara, YO
dc.contributor.authorPengo, T
dc.contributor.authorWidjaja, M
dc.contributor.authorKretschmer, S
dc.contributor.authorSwoger, J
dc.contributor.authorDjordjevic, S
dc.contributor.authorTurnbull, L
dc.contributor.authorWhitchurch, C
dc.contributor.authorMiyata, M
dc.contributor.authorMarti-Renom, MA
dc.contributor.authorLluch-Senar, M
dc.contributor.authorSerrano, L
dc.date.accessioned2020-12-22T05:29:24Z
dc.date.available2020-12-22T05:29:24Z
dc.date.issued2017-03-08
dc.identifierpii: ncomms14665
dc.identifier.citationTrussart, M., Yus, E., Martinez, S., Bau, D., Tahara, Y. O., Pengo, T., Widjaja, M., Kretschmer, S., Swoger, J., Djordjevic, S., Turnbull, L., Whitchurch, C., Miyata, M., Marti-Renom, M. A., Lluch-Senar, M. & Serrano, L. (2017). Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae. NATURE COMMUNICATIONS, 8 (1), https://doi.org/10.1038/ncomms14665.
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/11343/258300
dc.description.abstractDNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.titleDefined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae
dc.typeJournal Article
dc.identifier.doi10.1038/ncomms14665
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleNature Communications
melbourne.source.volume8
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1190699
melbourne.contributor.authorTrussart, Marie
dc.identifier.eissn2041-1723
melbourne.accessrightsOpen Access


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