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dc.contributor.authorWilliams, SP
dc.contributor.authorGould, CM
dc.contributor.authorNowell, CJ
dc.contributor.authorKarnezis, T
dc.contributor.authorAchen, MG
dc.contributor.authorSimpson, KJ
dc.contributor.authorStacker, SA
dc.date.accessioned2020-12-22T05:30:25Z
dc.date.available2020-12-22T05:30:25Z
dc.date.issued2017-03-01
dc.identifierpii: sdata20179
dc.identifier.citationWilliams, S. P., Gould, C. M., Nowell, C. J., Karnezis, T., Achen, M. G., Simpson, K. J. & Stacker, S. A. (2017). Data Descriptor: Systematic high-content genome-wide RNAi screens of endothelial cell migration and morphology. SCIENTIFIC DATA, 4 (1), https://doi.org/10.1038/sdata.2017.9.
dc.identifier.issn2052-4463
dc.identifier.urihttp://hdl.handle.net/11343/258303
dc.description.abstractMany cell types undergo migration during embryogenesis and disease. Endothelial cells line blood vessels and lymphatics, which migrate during development as part of angiogenesis, lymphangiogenesis and other types of vessel remodelling. These processes are also important in wound healing, cancer metastasis and cardiovascular conditions. However, the molecular control of endothelial cell migration is poorly understood. Here, we present a dataset containing siRNA screens that identify known and novel components of signalling pathways regulating migration of lymphatic endothelial cells. These components are compared to signalling in blood vascular endothelial cells. Further, using high-content microscopy, we captured a dataset of images of migrating cells following transfection with a genome-wide siRNA library. These datasets are suitable for the identification and analysis of genes involved in endothelial cell migration and morphology, and for computational approaches to identify signalling networks controlling the migratory response and integration of cell morphology, gene function and cell signaling. This may facilitate identification of protein targets for therapeutically modulating angiogenesis and lymphangiogenesis in the context of human disease.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.titleData Descriptor: Systematic high-content genome-wide RNAi screens of endothelial cell migration and morphology
dc.typeJournal Article
dc.identifier.doi10.1038/sdata.2017.9
melbourne.affiliation.departmentMedicine and Radiology
melbourne.affiliation.departmentSir Peter MacCallum Department of Oncology
melbourne.affiliation.departmentClinical Pathology
melbourne.source.titleScientific Data
melbourne.source.volume4
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1190960
melbourne.contributor.authorStacker, Steven
melbourne.contributor.authorAchen, Marc
melbourne.contributor.authorSimpson, Kaylene
melbourne.contributor.authorKarnezis, Tara
melbourne.contributor.authorWILLIAMS, STEVEN
dc.identifier.eissn2052-4463
melbourne.accessrightsOpen Access


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