Show simple item record

dc.contributor.authorFietz, ER
dc.contributor.authorKeenan, CR
dc.contributor.authorLopez-Campos, G
dc.contributor.authorTu, Y
dc.contributor.authorJohnstone, CN
dc.contributor.authorHarris, T
dc.contributor.authorStewart, AG
dc.date.accessioned2020-12-22T05:31:07Z
dc.date.available2020-12-22T05:31:07Z
dc.date.issued2017-03-06
dc.identifierpii: srep43774
dc.identifier.citationFietz, E. R., Keenan, C. R., Lopez-Campos, G., Tu, Y., Johnstone, C. N., Harris, T. & Stewart, A. G. (2017). Glucocorticoid resistance of migration and gene expression in a daughter MDA-MB-231 breast tumour cell line selected for high metastatic potential. SCIENTIFIC REPORTS, 7 (1), https://doi.org/10.1038/srep43774.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/258305
dc.description.abstractGlucocorticoids are commonly used to prevent chemotherapy-induced nausea and vomiting despite a lack of understanding of their direct effect on cancer progression. Recent studies suggest that glucocorticoids inhibit cancer cell migration. However, this action has not been investigated in estrogen receptor (ER)-negative breast tumour cells, although activation of the glucocorticoid receptor (GR) is associated with a worse prognosis in ER-negative breast cancers. In this study we have explored the effect of glucocorticoids on the migration of the ER-negative MDA-MB-231 human breast tumour cell line and the highly metastatic MDA-MB-231-HM.LNm5 cell line that was generated through in vivo cycling. We show for the first time that glucocorticoids inhibit 2- and 3-dimensional migration of MDA-MB-231 cells. Selection of cells for high metastatic potential resulted in a less migratory cell phenotype that was resistant to regulation by glucocorticoids and showed decreased GR receptor expression. The emergence of glucocorticoid resistance during metastatic selection may partly explain the apparent disparity between the clinical and in vitro evidence regarding the actions of glucocorticoids in cancer. These findings highlight the highly plastic nature of tumour cells, and underscore the need to more fully understand the direct effect of glucocorticoid treatment on different stages of metastatic progression.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.titleGlucocorticoid resistance of migration and gene expression in a daughter MDA-MB-231 breast tumour cell line selected for high metastatic potential
dc.typeJournal Article
dc.identifier.doi10.1038/srep43774
melbourne.affiliation.departmentPharmacology and Therapeutics
melbourne.affiliation.departmentClinical Pathology
melbourne.affiliation.departmentBio21
melbourne.source.titleScientific Reports
melbourne.source.volume7
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1191012
melbourne.contributor.authorJohnstone, Cameron
melbourne.contributor.authorLopez Campos, Guillermo
melbourne.contributor.authorStewart, Alastair
melbourne.contributor.authorKeenan, Christine
melbourne.contributor.authorFietz, Ebony
melbourne.contributor.authorTu, Yun
melbourne.contributor.authorHarris, Trudi
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record