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dc.contributor.authorSochalska, M
dc.contributor.authorOttina, E
dc.contributor.authorTuzlak, S
dc.contributor.authorHerzog, S
dc.contributor.authorHerold, M
dc.contributor.authorVillunger, A
dc.date.accessioned2020-12-22T06:03:38Z
dc.date.available2020-12-22T06:03:38Z
dc.date.issued2016-04-01
dc.identifierpii: cdd2015130
dc.identifier.citationSochalska, M., Ottina, E., Tuzlak, S., Herzog, S., Herold, M. & Villunger, A. (2016). Conditional knockdown of BCL2A1 reveals rate-limiting roles in BCR-dependent B-cell survival. CELL DEATH AND DIFFERENTIATION, 23 (4), pp.628-639. https://doi.org/10.1038/cdd.2015.130.
dc.identifier.issn1350-9047
dc.identifier.urihttp://hdl.handle.net/11343/258410
dc.description.abstractBcl2 family proteins control mitochondrial apoptosis and its members exert critical cell type and differentiation stage-specific functions, acting as barriers against autoimmunity or transformation. Anti-apoptotic Bcl2a1/Bfl1/A1 is frequently deregulated in different types of blood cancers in humans but its physiological role is poorly understood as quadruplication of the Bcl2a1 gene locus in mice hampers conventional gene targeting strategies. Transgenic overexpression of A1, deletion of the A1-a paralogue or constitutive knockdown in the hematopoietic compartment of mice by RNAi suggested rate-limiting roles in lymphocyte development, granulopoiesis and mast cell activation. Here we report on the consequences of conditional knockdown of A1 protein expression using a reverse transactivator (rtTA)-driven approach that highlights a critical role for this Bcl2 family member in the maintenance of mature B-cell homeostasis. Furthermore, we define the A1/Bim (Bcl-2 interacting mediator of cell death) axis as a target of key kinases mediating B-cell receptor (BCR)-dependent survival signals, such as, spleen tyrosine kinase (Syk) and Brutons tyrosine kinase (Btk). As such, A1 represents a putative target for the treatment of B-cell-related pathologies depending on hyperactivation of BCR-emanating survival signals and loss of A1 expression accounts, in part, for the pro-apoptotic effects of Syk- or Btk inhibitors that rely on the 'BH3-only' protein Bim for cell killing.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.titleConditional knockdown of BCL2A1 reveals rate-limiting roles in BCR-dependent B-cell survival
dc.typeJournal Article
dc.identifier.doi10.1038/cdd.2015.130
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleCell Death and Differentiation
melbourne.source.volume23
melbourne.source.issue4
melbourne.source.pages628-639
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1041885
melbourne.contributor.authorHerold, Marco
dc.identifier.eissn1476-5403
melbourne.accessrightsOpen Access


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