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dc.contributor.authorGhafar, A
dc.contributor.authorKoehler, AV
dc.contributor.authorHall, RS
dc.contributor.authorGauci, CG
dc.contributor.authorGasser, RB
dc.contributor.authorJabbar, A
dc.date.accessioned2021-01-06T01:33:01Z
dc.date.available2021-01-06T01:33:01Z
dc.date.issued2021
dc.identifierpii: microorganisms9010021
dc.identifier.citationGhafar, A., Koehler, A. V., Hall, R. S., Gauci, C. G., Gasser, R. B. & Jabbar, A. (2021). Targeted Next-Generation Sequencing and Informatics as an Effective Tool to Establish the Composition of Bovine Piroplasm Populations in Endemic Regions.. Microorganisms, 9 (1), https://doi.org/10.3390/microorganisms9010021.
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/11343/258568
dc.description.abstractProtists of the genera Babesia and Theileria (piroplasms) cause some of the most prevalent and debilitating diseases for bovines worldwide. In this study, we established and used a next-generation sequencing-informatic approach to explore the composition of Babesia and Theileria populations in cattle and water buffalo in a country (Pakistan) endemic for these pathogens. We collected individual blood samples from cattle (n = 212) and water buffalo (n = 154), extracted genomic DNAs, PCR-amplified the V4 hypervariable region of 18S small subunit rRNA gene from piroplasms, sequenced amplicons using Illumina technology, and then analysed data using bioinformatic platforms. The results revealed piroplasms in 68.9% (252/366) samples, with overall occurrence being markedly higher in cattle (85.8%) than in water buffaloes (45.5%). Babesia (B.) occultans and Theileria (T.) lestoquardi-like species were recorded for the first time in Pakistan, and, overall, T. annulata was most commonly detected (65.8%) followed by B. bovis (7.1%), B. bigemina (4.4%), and T. orientalis (0.5%), with the genetic variability within B. bovis being pronounced. The occurrence and composition of piroplasm species varied markedly across different agro-ecological zones. The high detection of T. annulata in asymptomatic animals suggested a relatively high level of endemic stability of tropical theileriosis in the bovine population.
dc.languageeng
dc.publisherMDPI AG
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleTargeted Next-Generation Sequencing and Informatics as an Effective Tool to Establish the Composition of Bovine Piroplasm Populations in Endemic Regions.
dc.typeJournal Article
dc.identifier.doi10.3390/microorganisms9010021
melbourne.affiliation.departmentVeterinary Biosciences
melbourne.source.titleMicroorganisms
melbourne.source.volume9
melbourne.source.issue1
melbourne.source.pages21-21
dc.rights.licenseCC BY
melbourne.elementsid1484682
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822421
melbourne.contributor.authorGhafar, Abdul
melbourne.contributor.authorGauci, Charles
melbourne.contributor.authorKoehler, Anson
melbourne.contributor.authorJabbar, Abdul
melbourne.contributor.authorGhafar, Abdul
melbourne.contributor.authorGasser, Robin
dc.identifier.eissn2076-2607
melbourne.accessrightsOpen Access


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