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dc.contributor.authorWike, CL
dc.contributor.authorGraves, HK
dc.contributor.authorHawkins, R
dc.contributor.authorGibson, MD
dc.contributor.authorFerdinand, MB
dc.contributor.authorZhang, T
dc.contributor.authorChen, Z
dc.contributor.authorHudson, DF
dc.contributor.authorOttesen, JJ
dc.contributor.authorPokier, MG
dc.contributor.authorSchumacher, J
dc.contributor.authorTyler, JK
dc.date.accessioned2021-02-03T23:51:16Z
dc.date.available2021-02-03T23:51:16Z
dc.date.issued2016-02-16
dc.identifier.citationWike, C. L., Graves, H. K., Hawkins, R., Gibson, M. D., Ferdinand, M. B., Zhang, T., Chen, Z., Hudson, D. F., Ottesen, J. J., Pokier, M. G., Schumacher, J. & Tyler, J. K. (2016). Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis. ELIFE, 5 (FEBRUARY2016), https://doi.org/10.7554/eLife.11402.
dc.identifier.issn2050-084X
dc.identifier.urihttp://hdl.handle.net/11343/258983
dc.description.abstractPhosphorylation of histone H3 threonine 118 (H3 T118ph) weakens histone DNA-contacts, disrupting the nucleosome structure. We show that Aurora-A mediated H3 T118ph occurs at pericentromeres and chromosome arms during prophase and is lost upon chromosome alignment. Expression of H3 T118E or H3 T118I (a SIN mutation that bypasses the need for the ATP-dependent nucleosome remodeler SWI/SNF) leads to mitotic problems including defects in spindle attachment, delayed cytokinesis, reduced chromatin packaging, cohesion loss, cohesin and condensin I loss in human cells. In agreement, overexpression of Aurora-A leads to increased H3 T118ph levels, causing cohesion loss, and reduced levels of cohesin and condensin I on chromatin. Normal levels of H3 T118ph are important because it is required for development in fruit flies. We propose that H3 T118ph alters the chromatin structure during specific phases of mitosis to promote timely condensin I and cohesin disassociation, which is essential for effective chromosome segregation.
dc.languageEnglish
dc.publisherELIFE SCIENCES PUBLICATIONS LTD
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleAurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis
dc.typeJournal Article
dc.identifier.doi10.7554/eLife.11402
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleeLife
melbourne.source.volume5
melbourne.source.issueFEBRUARY2016
dc.rights.licenseCC BY
melbourne.elementsid1051341
melbourne.contributor.authorHudson, Damien
dc.identifier.eissn2050-084X
melbourne.accessrightsOpen Access


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