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    Prospective Evaluation of ResistancePlus MG, a New Multiplex Quantitative PCR Assay for Detection of Mycoplasma genitalium and Macrolide Resistance

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    Author
    Tabrizi, SN; Su, J; Bradshaw, CS; Fairley, CK; Walker, S; Tan, LY; Mokany, E; Garland, SM
    Date
    2017-06-01
    Source Title
    Journal of Clinical Microbiology
    Publisher
    AMER SOC MICROBIOLOGY
    University of Melbourne Author/s
    Tabrizi, Sepehr; Wark, Suzanne; Bradshaw, Catriona
    Affiliation
    Obstetrics and Gynaecology
    Melbourne School of Population and Global Health
    Metadata
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    Document Type
    Journal Article
    Citations
    Tabrizi, S. N., Su, J., Bradshaw, C. S., Fairley, C. K., Walker, S., Tan, L. Y., Mokany, E. & Garland, S. M. (2017). Prospective Evaluation of ResistancePlus MG, a New Multiplex Quantitative PCR Assay for Detection of Mycoplasma genitalium and Macrolide Resistance. JOURNAL OF CLINICAL MICROBIOLOGY, 55 (6), pp.1915-1919. https://doi.org/10.1128/JCM.02312-16.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/259005
    DOI
    10.1128/JCM.02312-16
    Abstract
    Mycoplasma genitalium is a significant pathogen for which first-line treatment is becoming less effective due to increased resistance to macrolides. As conventional culture and antimicrobial susceptibility testing is not feasible for routine detection of this pathogen, molecular markers such as detection of mutations in the 23S rRNA gene have been described to predict resistance. Recently, a novel multiplex quantitative PCR (qPCR) assay, ResistancePlus MG, has been described for the simultaneous detection of Mycoplasma genitalium and macrolide resistance. In the current study, the clinical performance of the assay was evaluated on 1,089 consecutive urine and anogenital swab samples in symptomatic and asymptomatic male and female patients. Overall, 6.0% were positive for M. genitalium, with 63.1% having macrolide resistance-associated mutations. Compared to the laboratory-validated qPCR method targeting the 16S rRNA gene and Sanger sequencing to determine 23S rRNA mutations, the sensitivity and specificity of M. genitalium detection were 98.5% and 100% and for detection of macrolide resistance mutations were 100.0% and 96.2%, respectively. This assay offers a considerable advantage in clinical settings for M. genitalium testing by making the results of macrolide resistance and mutation analyses simultaneously available, which is increasingly important with escalating macrolide resistance.

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