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dc.contributor.authorTabrizi, SN
dc.contributor.authorSu, J
dc.contributor.authorBradshaw, CS
dc.contributor.authorFairley, CK
dc.contributor.authorWalker, S
dc.contributor.authorTan, LY
dc.contributor.authorMokany, E
dc.contributor.authorGarland, SM
dc.date.accessioned2021-02-03T23:58:10Z
dc.date.available2021-02-03T23:58:10Z
dc.date.issued2017-06-01
dc.identifierpii: JCM.02312-16
dc.identifier.citationTabrizi, S. N., Su, J., Bradshaw, C. S., Fairley, C. K., Walker, S., Tan, L. Y., Mokany, E. & Garland, S. M. (2017). Prospective Evaluation of ResistancePlus MG, a New Multiplex Quantitative PCR Assay for Detection of Mycoplasma genitalium and Macrolide Resistance. JOURNAL OF CLINICAL MICROBIOLOGY, 55 (6), pp.1915-1919. https://doi.org/10.1128/JCM.02312-16.
dc.identifier.issn0095-1137
dc.identifier.urihttp://hdl.handle.net/11343/259005
dc.description.abstractMycoplasma genitalium is a significant pathogen for which first-line treatment is becoming less effective due to increased resistance to macrolides. As conventional culture and antimicrobial susceptibility testing is not feasible for routine detection of this pathogen, molecular markers such as detection of mutations in the 23S rRNA gene have been described to predict resistance. Recently, a novel multiplex quantitative PCR (qPCR) assay, ResistancePlus MG, has been described for the simultaneous detection of Mycoplasma genitalium and macrolide resistance. In the current study, the clinical performance of the assay was evaluated on 1,089 consecutive urine and anogenital swab samples in symptomatic and asymptomatic male and female patients. Overall, 6.0% were positive for M. genitalium, with 63.1% having macrolide resistance-associated mutations. Compared to the laboratory-validated qPCR method targeting the 16S rRNA gene and Sanger sequencing to determine 23S rRNA mutations, the sensitivity and specificity of M. genitalium detection were 98.5% and 100% and for detection of macrolide resistance mutations were 100.0% and 96.2%, respectively. This assay offers a considerable advantage in clinical settings for M. genitalium testing by making the results of macrolide resistance and mutation analyses simultaneously available, which is increasingly important with escalating macrolide resistance.
dc.languageEnglish
dc.publisherAMER SOC MICROBIOLOGY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleProspective Evaluation of ResistancePlus MG, a New Multiplex Quantitative PCR Assay for Detection of Mycoplasma genitalium and Macrolide Resistance
dc.typeJournal Article
dc.identifier.doi10.1128/JCM.02312-16
melbourne.affiliation.departmentObstetrics and Gynaecology
melbourne.affiliation.departmentMelbourne School of Population and Global Health
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleJournal of Clinical Microbiology
melbourne.source.volume55
melbourne.source.issue6
melbourne.source.pages1915-1919
dc.rights.licenseCC BY
melbourne.elementsid1196976
melbourne.contributor.authorTabrizi, Sepehr
melbourne.contributor.authorWark, Suzanne
melbourne.contributor.authorBradshaw, Catriona
melbourne.contributor.authorSu, Jenny
dc.identifier.eissn1098-660X
melbourne.accessrightsOpen Access


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