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dc.contributor.authorMiles, LB
dc.contributor.authorMizoguchi, T
dc.contributor.authorKikuchi, Y
dc.contributor.authorVerkade, H
dc.date.accessioned2021-02-04T00:02:57Z
dc.date.available2021-02-04T00:02:57Z
dc.date.issued2017-05-15
dc.identifierpii: bio.021899
dc.identifier.citationMiles, L. B., Mizoguchi, T., Kikuchi, Y. & Verkade, H. (2017). A role for planar cell polarity during early endoderm morphogenesis. BIOLOGY OPEN, 6 (5), pp.531-539. https://doi.org/10.1242/bio.021899.
dc.identifier.issn2046-6390
dc.identifier.urihttp://hdl.handle.net/11343/259018
dc.description.abstractThe zebrafish endoderm begins to develop at gastrulation stages as a monolayer of cells. The behaviour of the endoderm during gastrulation stages is well understood. However, knowledge of the morphogenic movements of the endoderm during somitogenesis stages, as it forms a mesenchymal rod, is lacking. Here we characterise endodermal development during somitogenesis stages, and describe the morphogenic movements as the endoderm transitions from a monolayer of cells into a mesenchymal endodermal rod. We demonstrate that, unlike the overlying mesoderm, endodermal cells are not polarised during their migration to the midline at early somitogenesis stages. Specifically, we describe the stage at which endodermal cells begin to leave the monolayer, a process we have termed 'midline aggregation'. The planar cell polarity (PCP) signalling pathway is known to regulate mesodermal and ectodermal cell convergence towards the dorsal midline. However, a role for PCP signalling in endoderm migration to the midline during somitogenesis stages has not been established. In this report, we investigate the role for PCP signalling in multiple phases of endoderm development during somitogenesis stages. Our data exclude involvement of PCP signalling in endodermal cells as they leave the monolayer.
dc.languageEnglish
dc.publisherCOMPANY OF BIOLOGISTS LTD
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleA role for planar cell polarity during early endoderm morphogenesis
dc.typeJournal Article
dc.identifier.doi10.1242/bio.021899
melbourne.affiliation.departmentBiochemistry and Molecular Biology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleBiology Open
melbourne.source.volume6
melbourne.source.issue5
melbourne.source.pages531-539
dc.rights.licenseCC BY
melbourne.elementsid1197289
melbourne.contributor.authorVerkade, Heather
dc.identifier.eissn2046-6390
melbourne.accessrightsOpen Access


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