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dc.contributor.authorChakravorty, A
dc.contributor.authorAwad, MM
dc.contributor.authorHiscox, TJ
dc.contributor.authorCheung, JK
dc.contributor.authorCarter, GP
dc.contributor.authorChoo, JM
dc.contributor.authorLyras, D
dc.contributor.authorRood, JI
dc.date.accessioned2021-02-04T00:09:01Z
dc.date.available2021-02-04T00:09:01Z
dc.date.issued2011-07-29
dc.identifierpii: PONE-D-11-09035
dc.identifier.citationChakravorty, A., Awad, M. M., Hiscox, T. J., Cheung, J. K., Carter, G. P., Choo, J. M., Lyras, D. & Rood, J. I. (2011). The Cysteine Protease alpha-Clostripain is Not Essential for the Pathogenesis of Clostridium perfringens-Mediated Myonecrosis. PLOS ONE, 6 (7), https://doi.org/10.1371/journal.pone.0022762.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/259041
dc.description.abstractClostridium perfringens is the causative agent of clostridial myonecrosis or gas gangrene and produces many different extracellular toxins and enzymes, including the cysteine protease α-clostripain. Mutation of the α-clostripain structural gene, ccp, alters the turnover of secreted extracellular proteins in C. perfringens, but the role of α-clostripain in disease pathogenesis is not known. We insertionally inactivated the ccp gene C. perfringens strain 13 using TargeTron technology, constructing a strain that was no longer proteolytic on skim milk agar. Quantitative protease assays confirmed the absence of extracellular protease activity, which was restored by complementation with the wild-type ccp gene. The role of α-clostripain in virulence was assessed by analysing the isogenic wild-type, mutant and complemented strains in a mouse myonecrosis model. The results showed that although α-clostripain was the major extracellular protease, mutation of the ccp gene did not alter either the progression or the development of disease. These results do not rule out the possibility that this extracellular enzyme may still have a role in the early stages of the disease process.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleThe Cysteine Protease alpha-Clostripain is Not Essential for the Pathogenesis of Clostridium perfringens-Mediated Myonecrosis
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0022762
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titlePLoS One
melbourne.source.volume6
melbourne.source.issue7
dc.rights.licenseCC BY
melbourne.elementsid1197199
melbourne.contributor.authorCarter, Glen
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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