Cell culture: complications due to mechanical release of ATP and activation of purinoceptors
AuthorBurnstock, G; Knight, GE
Source TitleCell and Tissue Research
University of Melbourne Author/sBurnstock, Geoffrey
AffiliationPharmacology and Therapeutics
Document TypeJournal Article
CitationsBurnstock, G. & Knight, G. E. (2017). Cell culture: complications due to mechanical release of ATP and activation of purinoceptors. CELL AND TISSUE RESEARCH, 370 (1), pp.1-11. https://doi.org/10.1007/s00441-017-2618-8.
Access StatusOpen Access
There is abundant evidence that ATP (adenosine 5'-triphosphate) is released from a variety of cultured cells in response to mechanical stimulation. The release mechanism involved appears to be a combination of vesicular exocytosis and connexin and pannexin hemichannels. Purinergic receptors on cultured cells mediate both short-term purinergic signalling of secretion and long-term (trophic) signalling such as proliferation, migration, differentiation and apoptosis. We aim in this review to bring to the attention of non-purinergic researchers using tissue culture that the release of ATP in response to mechanical stress evoked by the unavoidable movement of the cells acting on functional purinergic receptors on the culture cells is likely to complicate the interpretation of their data.
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