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dc.contributor.authorLeang, S-K
dc.contributor.authorHurt, AC
dc.date.accessioned2021-02-04T00:25:47Z
dc.date.available2021-02-04T00:25:47Z
dc.date.issued2017-04-01
dc.identifier.citationLeang, S. -K. & Hurt, A. C. (2017). Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2017 (122), https://doi.org/10.3791/55570.
dc.identifier.issn1940-087X
dc.identifier.urihttp://hdl.handle.net/11343/259106
dc.description.abstractThe neuraminidase (NA) inhibitors are the only class of antivirals approved for the treatment and prophylaxis of influenza that are effective against currently circulating strains. In addition to their use in treating seasonal influenza, the NA inhibitors have been stockpiled by a number of countries for use in the event of a pandemic. It is therefore important to monitor the susceptibility of circulating influenza viruses to this class of antivirals. There are different types of assays that can be used to assess the susceptibility of influenza viruses to the NA inhibitors, but the enzyme inhibition assays using either a fluorescent substrate or a chemiluminescent substrate are the most widely used and recommended. This protocol describes the use of a fluorescence-based assay to assess influenza virus susceptibility to NA inhibitors. The assay is based on the NA enzyme cleaving the 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid (MUNANA) substrate to release the fluorescent product 4-methylumbelliferone (4-MU). Therefore, the inhibitory effect of an NA inhibitor on the influenza virus NA is determined based on the concentration of the NA inhibitor that is required to reduce 50% of the NA activity, given as an IC50 value.
dc.languageEnglish
dc.publisherJOURNAL OF VISUALIZED EXPERIMENTS
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.titleFluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
dc.typeJournal Article
dc.identifier.doi10.3791/55570
melbourne.affiliation.departmentMelbourne School of Population and Global Health
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleJournal of Visualized Experiments
melbourne.source.volume2017
melbourne.source.issue122
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1207805
melbourne.contributor.authorHurt, Aeron
dc.identifier.eissn1940-087X
melbourne.accessrightsOpen Access


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