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dc.contributor.authorSchlissel, MS
dc.contributor.authorCorcoran, LM
dc.contributor.authorBaltimore, D
dc.date.accessioned2021-02-04T00:28:37Z
dc.date.available2021-02-04T00:28:37Z
dc.date.issued1991-03-01
dc.identifier.citationSchlissel, M. S., Corcoran, L. M. & Baltimore, D. (1991). Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription.. J Exp Med, 173 (3), pp.711-720. https://doi.org/10.1084/jem.173.3.711.
dc.identifier.issn0022-1007
dc.identifier.urihttp://hdl.handle.net/11343/259117
dc.description.abstractVirus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to study both the possible relationship between these processes in cultured pre-B cells and the role played by heavy (H) chain (mu) protein in regulating gene rearrangement. Our analysis of pre-B cell cultures representing various stages of maturity revealed that transcription of each germline Ig locus precedes or is coincident with its rearrangement. Cell lines containing one functional rearranged H chain allele, however, continue to transcribe and to rearrange the allelic, unrearranged H chain locus. These cell lines appear to initiate but not terminate rearrangement events and therefore provide information about the requirements for activating rearrangement but not about allelic exclusion mechanisms.
dc.languageeng
dc.publisherRockefeller University Press
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.titleVirus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription.
dc.typeJournal Article
dc.identifier.doi10.1084/jem.173.3.711
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleJournal of Experimental Medicine
melbourne.source.volume173
melbourne.source.issue3
melbourne.source.pages711-720
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1208347
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118835
melbourne.contributor.authorCorcoran, Lynn
dc.identifier.eissn1540-9538
melbourne.accessrightsOpen Access


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