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dc.contributor.authorCampbell, GR
dc.contributor.authorUehara, Y
dc.contributor.authorMark, G
dc.contributor.authorBurnstock, G
dc.date.accessioned2021-02-04T00:35:06Z
dc.date.available2021-02-04T00:35:06Z
dc.date.issued1971-04
dc.identifier.citationCampbell, G. R., Uehara, Y., Mark, G. & Burnstock, G. (1971). Fine structure of smooth muscle cells grown in tissue culture.. J Cell Biol, 49 (1), pp.21-34. https://doi.org/10.1083/jcb.49.1.21.
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/11343/259139
dc.description.abstractThe fine structure of smooth muscle cells of the embryo chicken gizzard cultured in monolayer was studied by phase-contrast optics and electron microscopy. The smooth muscle cells were irregular in shape, but tended to be elongate. The nucleus usually contained prominent nucleoli and was large in relation to the cell body. When fixed with glutaraldehyde, three different types of filaments were noted in the cytoplasm: thick (150-250 A in diameter) and thin (30-80 A in diameter) myofilaments, many of which were arranged in small bundles throughout the cytoplasm and which were usually associated with dark bodies; and filaments with a diameter of 80-110 A which were randomly orientated and are not regarded as myofilaments. Some of the aggregated ribosomes were helically arranged. Mitochondria, Golgi apparatus, and dilated rough endoplasmic reticulum were prominent. In contrast to in vivo muscle cells, micropinocytotic vesicles along the cell membrane were rare and dense areas were usually confined to cell membrane infoldings. These cells are compared to in vivo embryonic smooth muscle and adult muscle after treatment with estrogen. Monolayers of cultured smooth muscle will be of particular value in relating ultrastructural features to functional observations on the same cells.
dc.languageeng
dc.publisherRockefeller University Press
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.titleFine structure of smooth muscle cells grown in tissue culture.
dc.typeJournal Article
dc.identifier.doi10.1083/jcb.49.1.21
melbourne.affiliation.departmentPharmacology and Therapeutics
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleThe Journal of Cell Biology
melbourne.source.volume49
melbourne.source.issue1
melbourne.source.pages21-34
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1198710
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108199
melbourne.contributor.authorBurnstock, Geoffrey
dc.identifier.eissn1540-8140
melbourne.accessrightsOpen Access


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