ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2X(5) receptor on satellite cells

Download
Author
Ryten, M; Dunn, PM; Neary, JT; Burnstock, GDate
2002-07-22Source Title
The Journal of Cell BiologyPublisher
ROCKEFELLER UNIV PRESSUniversity of Melbourne Author/s
Burnstock, GeoffreyAffiliation
Pharmacology and TherapeuticsMetadata
Show full item recordDocument Type
Journal ArticleCitations
Ryten, M., Dunn, P. M., Neary, J. T. & Burnstock, G. (2002). ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2X(5) receptor on satellite cells. JOURNAL OF CELL BIOLOGY, 158 (2), pp.345-355. https://doi.org/10.1083/jcb.200202025.Access Status
Open AccessAbstract
ATP is well known for its role as an intracellular energy source. However, there is increasing awareness of its role as an extracellular messenger molecule (Burnstock, 1997). Although evidence for the presence of receptors for extracellular ATP on skeletal myoblasts was first published in 1983 (Kolb and Wakelam), their physiological function has remained unclear. In this paper we used primary cultures of rat skeletal muscle satellite cells to investigate the role of purinergic signaling in muscle formation. Using immunocytochemistry, RT-PCR, and electrophysiology, we demonstrate that the ionotropic P2X5 receptor is present on satellite cells and that activation of a P2X receptor inhibits proliferation, stimulates expression of markers of muscle cell differentiation, including myogenin, p21, and myosin heavy chain, and increases the rate of myotube formation. Furthermore, we demonstrate that ATP application results in a significant and rapid increase in the phosphorylation of MAPKs, particularly p38, and that inhibition of p38 activity can prevent the effect of ATP on cell number. These results not only demonstrate the existence of a novel regulator of skeletal muscle differentiation, namely ATP, but also a new role for ionotropic P2X receptors in the control of cell fate.
Export Reference in RIS Format
Endnote
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
Refworks
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References