University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Affiliates
  • Bio21
  • Bio21 - Research Publications
  • View Item
  • Minerva Access
  • Affiliates
  • Bio21
  • Bio21 - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    CD155/PVR plays a key role in cell motility during tumor cell invasion and migration.

    Thumbnail
    Download
    Published version (1.472Mb)

    Citations
    Scopus
    Web of Science
    Altmetric
    147
    140
    Author
    Sloan, KE; Eustace, BK; Stewart, JK; Zehetmeier, C; Torella, C; Simeone, M; Roy, JE; Unger, C; Louis, DN; Ilag, LL; ...
    Date
    2004-10-07
    Source Title
    BMC Cancer
    Publisher
    Springer Science and Business Media LLC
    University of Melbourne Author/s
    ILAG, LEODEVICO
    Affiliation
    Bio21
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Sloan, K. E., Eustace, B. K., Stewart, J. K., Zehetmeier, C., Torella, C., Simeone, M., Roy, J. E., Unger, C., Louis, D. N., Ilag, L. L. & Jay, D. G. (2004). CD155/PVR plays a key role in cell motility during tumor cell invasion and migration.. BMC Cancer, 4 (1), pp.73-. https://doi.org/10.1186/1471-2407-4-73.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/259196
    DOI
    10.1186/1471-2407-4-73
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC524493
    Abstract
    BACKGROUND: Invasion is an important early step of cancer metastasis that is not well understood. Developing therapeutics to limit metastasis requires the identification and validation of candidate proteins necessary for invasion and migration. METHODS: We developed a functional proteomic screen to identify mediators of tumor cell invasion. This screen couples Fluorophore Assisted Light Inactivation (FALI) to a scFv antibody library to systematically inactivate surface proteins expressed by human fibrosarcoma cells followed by a high-throughput assessment of transwell invasion. RESULTS: Using this screen, we have identified CD155 (the poliovirus receptor) as a mediator of tumor cell invasion through its role in migration. Knockdown of CD155 by FALI or by RNAi resulted in a significant decrease in transwell migration of HT1080 fibrosarcoma cells towards a serum chemoattractant. CD155 was found to be highly expressed in multiple cancer cell lines and primary tumors including glioblastoma (GBM). Knockdown of CD155 also decreased migration of U87MG GBM cells. CD155 is recruited to the leading edge of migrating cells where it colocalizes with actin and alphav-integrin, known mediators of motility and adhesion. Knockdown of CD155 also altered cellular morphology, resulting in cells that were larger and more elongated than controls when plated on a Matrigel substrate. CONCLUSION: These results implicate a role for CD155 in mediating tumor cell invasion and migration and suggest that CD155 may contribute to tumorigenesis.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52443]
    • Bio21 - Research Publications [308]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors