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dc.contributor.authorSloan, KE
dc.contributor.authorEustace, BK
dc.contributor.authorStewart, JK
dc.contributor.authorZehetmeier, C
dc.contributor.authorTorella, C
dc.contributor.authorSimeone, M
dc.contributor.authorRoy, JE
dc.contributor.authorUnger, C
dc.contributor.authorLouis, DN
dc.contributor.authorIlag, LL
dc.contributor.authorJay, DG
dc.date.accessioned2021-02-04T00:48:28Z
dc.date.available2021-02-04T00:48:28Z
dc.date.issued2004-10-07
dc.identifierpii: 1471-2407-4-73
dc.identifier.citationSloan, K. E., Eustace, B. K., Stewart, J. K., Zehetmeier, C., Torella, C., Simeone, M., Roy, J. E., Unger, C., Louis, D. N., Ilag, L. L. & Jay, D. G. (2004). CD155/PVR plays a key role in cell motility during tumor cell invasion and migration.. BMC Cancer, 4 (1), pp.73-. https://doi.org/10.1186/1471-2407-4-73.
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/11343/259196
dc.description.abstractBACKGROUND: Invasion is an important early step of cancer metastasis that is not well understood. Developing therapeutics to limit metastasis requires the identification and validation of candidate proteins necessary for invasion and migration. METHODS: We developed a functional proteomic screen to identify mediators of tumor cell invasion. This screen couples Fluorophore Assisted Light Inactivation (FALI) to a scFv antibody library to systematically inactivate surface proteins expressed by human fibrosarcoma cells followed by a high-throughput assessment of transwell invasion. RESULTS: Using this screen, we have identified CD155 (the poliovirus receptor) as a mediator of tumor cell invasion through its role in migration. Knockdown of CD155 by FALI or by RNAi resulted in a significant decrease in transwell migration of HT1080 fibrosarcoma cells towards a serum chemoattractant. CD155 was found to be highly expressed in multiple cancer cell lines and primary tumors including glioblastoma (GBM). Knockdown of CD155 also decreased migration of U87MG GBM cells. CD155 is recruited to the leading edge of migrating cells where it colocalizes with actin and alphav-integrin, known mediators of motility and adhesion. Knockdown of CD155 also altered cellular morphology, resulting in cells that were larger and more elongated than controls when plated on a Matrigel substrate. CONCLUSION: These results implicate a role for CD155 in mediating tumor cell invasion and migration and suggest that CD155 may contribute to tumorigenesis.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleCD155/PVR plays a key role in cell motility during tumor cell invasion and migration.
dc.typeJournal Article
dc.identifier.doi10.1186/1471-2407-4-73
melbourne.affiliation.departmentBio21
melbourne.affiliation.facultyAffiliate
melbourne.source.titleBMC Cancer
melbourne.source.volume4
melbourne.source.issue1
melbourne.source.pages73-
dc.rights.licenseCC BY
melbourne.elementsid1206228
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC524493
melbourne.contributor.authorILAG, LEODEVICO
dc.identifier.eissn1471-2407
melbourne.accessrightsOpen Access


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