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dc.contributor.authorGiles, C
dc.contributor.authorTakechi, R
dc.contributor.authorMellett, NA
dc.contributor.authorMeikle, PJ
dc.contributor.authorDhaliwal, S
dc.contributor.authorMamo, JC
dc.date.accessioned2021-02-04T00:51:45Z
dc.date.available2021-02-04T00:51:45Z
dc.date.issued2016-12-01
dc.identifierpii: PONE-D-16-30995
dc.identifier.citationGiles, C., Takechi, R., Mellett, N. A., Meikle, P. J., Dhaliwal, S. & Mamo, J. C. (2016). The Effects of Long-Term Saturated Fat Enriched Diets on the Brain Lipidome. PLOS ONE, 11 (12), https://doi.org/10.1371/journal.pone.0166964.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/259211
dc.description.abstractThe brain is highly enriched in lipids, where they influence neurotransmission, synaptic plasticity and inflammation. Non-pathological modulation of the brain lipidome has not been previously reported and few studies have investigated the interplay between plasma lipid homeostasis relative to cerebral lipids. This study explored whether changes in plasma lipids induced by chronic consumption of a well-tolerated diet enriched in saturated fatty acids (SFA) was associated with parallel changes in cerebral lipid homeostasis. Male C57Bl/6 mice were fed regular chow or the SFA diet for six months. Plasma, hippocampus (HPF) and cerebral cortex (CTX) lipids were analysed by LC-ESI-MS/MS. A total of 348 lipid species were determined, comprising 25 lipid classes. The general abundance of HPF and CTX lipids was comparable in SFA fed mice versus controls, despite substantial differences in plasma lipid-class abundance. However, significant differences in 50 specific lipid species were identified as a consequence of SFA treatment, restricted to phosphatidylcholine (PC), phosphatidylethanolamine (PE), alkyl-PC, alkenyl-PC, alkyl-PE, alkenyl-PE, cholesterol ester (CE), diacylglycerol (DG), phosphatidylinositol (PI) and phosphatidylserine (PS) classes. Partial least squares regression of the HPF/CTX lipidome versus plasma lipidome revealed the plasma lipidome could account for a substantial proportion of variation. The findings demonstrate that cerebral abundance of specific lipid species is strongly associated with plasma lipid homeostasis.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleThe Effects of Long-Term Saturated Fat Enriched Diets on the Brain Lipidome
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0166964
melbourne.affiliation.departmentBio21
melbourne.affiliation.departmentMelbourne Medical School
melbourne.affiliation.facultyAffiliate
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titlePLoS One
melbourne.source.volume11
melbourne.source.issue12
dc.rights.licenseCC BY
melbourne.elementsid1208029
melbourne.contributor.authorMeikle, Peter
melbourne.contributor.authorGiles, Corey
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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