Show simple item record

dc.contributor.authorBarber, MN
dc.contributor.authorRisis, S
dc.contributor.authorYang, C
dc.contributor.authorMeikle, PJ
dc.contributor.authorStaples, M
dc.contributor.authorFebbraio, MA
dc.contributor.authorBruce, CR
dc.date.accessioned2021-02-04T00:55:55Z
dc.date.available2021-02-04T00:55:55Z
dc.date.issued2012-07-25
dc.identifierpii: PONE-D-12-02596
dc.identifier.citationBarber, M. N., Risis, S., Yang, C., Meikle, P. J., Staples, M., Febbraio, M. A. & Bruce, C. R. (2012). Plasma Lysophosphatidylcholine Levels Are Reduced in Obesity and Type 2 Diabetes. PLOS ONE, 7 (7), https://doi.org/10.1371/journal.pone.0041456.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/259226
dc.description.abstractBACKGROUND: Obesity and type 2 diabetes (T2DM) are associated with increased circulating free fatty acids and triacylglycerols. However, very little is known about specific molecular lipid species associated with these diseases. In order to gain further insight into this, we performed plasma lipidomic analysis in a rodent model of obesity and insulin resistance as well as in lean, obese and obese individuals with T2DM. METHODOLOGY/PRINCIPAL FINDINGS: Lipidomic analysis using liquid chromatography coupled to mass spectrometry revealed marked changes in the plasma of 12 week high fat fed mice. Although a number of triacylglycerol and diacylglycerol species were elevated along with of a number of sphingolipids, a particularly interesting finding was the high fat diet (HFD)-induced reduction in lysophosphatidylcholine (LPC) levels. As liver, skeletal muscle and adipose tissue play an important role in metabolism, we next determined whether the HFD altered LPCs in these tissues. In contrast to our findings in plasma, only very modest changes in tissue LPCs were noted. To determine when the change in plasma LPCs occurred in response to the HFD, mice were studied after 1, 3 and 6 weeks of HFD. The HFD caused rapid alterations in plasma LPCs with most changes occurring within the first week. Consistent with our rodent model, data from our small human cohort showed a reduction in a number of LPC species in obese and obese individuals with T2DM. Interestingly, no differences were found between the obese otherwise healthy individuals and the obese T2DM patients. CONCLUSION: Irrespective of species, our lipidomic profiling revealed a generalized decrease in circulating LPC species in states of obesity. Moreover, our data indicate that diet and adiposity, rather than insulin resistance or diabetes per se, play an important role in altering the plasma LPC profile.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.titlePlasma Lysophosphatidylcholine Levels Are Reduced in Obesity and Type 2 Diabetes
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0041456
melbourne.affiliation.departmentBio21
melbourne.affiliation.facultyAffiliate
melbourne.source.titlePLoS One
melbourne.source.volume7
melbourne.source.issue7
dc.rights.licenseCC BY
melbourne.elementsid1208059
melbourne.contributor.authorMeikle, Peter
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record