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dc.contributor.authorHewitt, AW
dc.contributor.authorJanuar, V
dc.contributor.authorSexton-Oates, A
dc.contributor.authorJoo, JE
dc.contributor.authorFranchina, M
dc.contributor.authorWang, JJ
dc.contributor.authorLiang, H
dc.contributor.authorCraig, JE
dc.contributor.authorSaffery, R
dc.date.accessioned2021-02-04T01:00:25Z
dc.date.available2021-02-04T01:00:25Z
dc.date.issued2017-04-13
dc.identifierpii: srep46330
dc.identifier.citationHewitt, A. W., Januar, V., Sexton-Oates, A., Joo, J. E., Franchina, M., Wang, J. J., Liang, H., Craig, J. E. & Saffery, R. (2017). DNA methylation landscape of ocular tissue relative to matched peripheral blood. SCIENTIFIC REPORTS, 7 (1), https://doi.org/10.1038/srep46330.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/259242
dc.description.abstractEpigenetic variation is implicated in a range of non-communicable diseases, including those of the eye. However, investigating the role of epigenetic variation in central tissues, such as eye or brain, remains problematic and peripheral tissues are often used as surrogates. In this study, matched human blood and eye tissue (n = 8) were obtained post-mortem and DNA methylation profiling performed on blood, neurosensory retina, retinal pigment epithelium (RPE)/choroid and optic nerve tissue using the Illumina Infinium HumanMethylation450 platform. Unsupervised clustering and principal components analysis revealed tissue of origin as the main driver of methylation variation. Despite this, there was a strong correlation of methylation profiles between tissues with >255,000 CpG sites found to have similar methylation levels. An additional ~16,000 show similarity across ocular tissues only. A small proportion of probes showing inter-individual variation in blood co-varied with eye tissues within individuals, however much of this variation may be genetically driven. An improved understanding of the epigenetic landscape of the eye will have important implications for understanding eye disease. Despite a generally high correlation irrespective of origin, tissue type is the major driver of methylation variation, with only limited covariation between blood and any specific ocular tissue.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleDNA methylation landscape of ocular tissue relative to matched peripheral blood
dc.typeJournal Article
dc.identifier.doi10.1038/srep46330
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.affiliation.departmentClinical Pathology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleScientific Reports
melbourne.source.volume7
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1206103
melbourne.contributor.authorJoo, Ji Hoon
melbourne.contributor.authorSaffery, Richard
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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