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dc.contributor.authorSrivastava, A
dc.contributor.authorKowalski, GM
dc.contributor.authorCallahan, DL
dc.contributor.authorMeikle, PJ
dc.contributor.authorCreek, DJ
dc.date.accessioned2021-02-04T01:03:27Z
dc.date.available2021-02-04T01:03:27Z
dc.date.issued2016-12-01
dc.identifierpii: metabo6040032
dc.identifier.citationSrivastava, A., Kowalski, G. M., Callahan, D. L., Meikle, P. J. & Creek, D. J. (2016). Strategies for Extending Metabolomics Studies with Stable Isotope Labelling and Fluxomics. METABOLITES, 6 (4), https://doi.org/10.3390/metabo6040032.
dc.identifier.issn2218-1989
dc.identifier.urihttp://hdl.handle.net/11343/259252
dc.description.abstractThis is a perspective from the peer session on stable isotope labelling and fluxomics at the Australian & New Zealand Metabolomics Conference (ANZMET) held from 30 March to 1 April 2016 at La Trobe University, Melbourne, Australia. This report summarizes the key points raised in the peer session which focused on the advantages of using stable isotopes in modern metabolomics and the challenges in conducting flux analyses. The session highlighted the utility of stable isotope labelling in generating reference standards for metabolite identification, absolute quantification, and in the measurement of the dynamic activity of metabolic pathways. The advantages and disadvantages of different approaches of fluxomics analyses including flux balance analysis, metabolic flux analysis and kinetic flux profiling were also discussed along with the use of stable isotope labelling in in vivo dynamic metabolomics. A number of crucial technical considerations for designing experiments and analyzing data with stable isotope labelling were discussed which included replication, instrumentation, methods of labelling, tracer dilution and data analysis. This report reflects the current viewpoint on the use of stable isotope labelling in metabolomics experiments, identifying it as a great tool with the potential to improve biological interpretation of metabolomics data in a number of ways.
dc.languageEnglish
dc.publisherMDPI
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleStrategies for Extending Metabolomics Studies with Stable Isotope Labelling and Fluxomics
dc.typeJournal Article
dc.identifier.doi10.3390/metabo6040032
melbourne.affiliation.departmentSchool of BioSciences
melbourne.affiliation.departmentBio21
melbourne.affiliation.facultyScience
melbourne.affiliation.facultyAffiliate
melbourne.source.titleMetabolites
melbourne.source.volume6
melbourne.source.issue4
dc.rights.licenseCC BY
melbourne.elementsid1208035
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192438
melbourne.contributor.authorMeikle, Peter
melbourne.contributor.authorCALLAHAN, DAMIEN
dc.identifier.eissn2218-1989
melbourne.accessrightsOpen Access


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