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dc.contributor.authorRodriguez-Andres, J
dc.contributor.authorRani, S
dc.contributor.authorVarjak, M
dc.contributor.authorChase-Topping, ME
dc.contributor.authorBeck, MH
dc.contributor.authorFerguson, MC
dc.contributor.authorSchnettler, E
dc.contributor.authorFragkoudis, R
dc.contributor.authorBarry, G
dc.contributor.authorMerits, A
dc.contributor.authorFazakerley, JK
dc.contributor.authorStrand, MR
dc.contributor.authorKohl, A
dc.date.accessioned2021-02-04T01:08:39Z
dc.date.available2021-02-04T01:08:39Z
dc.date.issued2012-11-01
dc.identifierpii: PPATHOGENS-D-12-01166
dc.identifier.citationRodriguez-Andres, J., Rani, S., Varjak, M., Chase-Topping, M. E., Beck, M. H., Ferguson, M. C., Schnettler, E., Fragkoudis, R., Barry, G., Merits, A., Fazakerley, J. K., Strand, M. R. & Kohl, A. (2012). Phenoloxidase Activity Acts as a Mosquito Innate Immune Response against Infection with Semliki Forest Virus. PLOS PATHOGENS, 8 (11), https://doi.org/10.1371/journal.ppat.1002977.
dc.identifier.issn1553-7366
dc.identifier.urihttp://hdl.handle.net/11343/259274
dc.description.abstractSeveral components of the mosquito immune system including the RNA interference (RNAi), JAK/STAT, Toll and IMD pathways have previously been implicated in controlling arbovirus infections. In contrast, the role of the phenoloxidase (PO) cascade in mosquito antiviral immunity is unknown. Here we show that conditioned medium from the Aedes albopictus-derived U4.4 cell line contains a functional PO cascade, which is activated by the bacterium Escherichia coli and the arbovirus Semliki Forest virus (SFV) (Togaviridae; Alphavirus). Production of recombinant SFV expressing the PO cascade inhibitor Egf1.0 blocked PO activity in U4.4 cell- conditioned medium, which resulted in enhanced spread of SFV. Infection of adult female Aedes aegypti by feeding mosquitoes a bloodmeal containing Egf1.0-expressing SFV increased virus replication and mosquito mortality. Collectively, these results suggest the PO cascade of mosquitoes plays an important role in immune defence against arboviruses.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.titlePhenoloxidase Activity Acts as a Mosquito Innate Immune Response against Infection with Semliki Forest Virus
dc.typeJournal Article
dc.identifier.doi10.1371/journal.ppat.1002977
melbourne.affiliation.departmentVeterinary Biosciences
melbourne.affiliation.facultyCollected Works
melbourne.source.titlePLoS Pathogens
melbourne.source.volume8
melbourne.source.issue11
dc.rights.licenseCC BY
melbourne.elementsid1053747
melbourne.contributor.authorRodriguez-Andres, Julio
melbourne.contributor.authorFazakerley, John
dc.identifier.eissn1553-7374
melbourne.accessrightsOpen Access


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