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    Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology

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    Author
    Tabrizi, SN; Tan, LY; Walker, S; Twin, J; Poljak, M; Bradshaw, CS; Fairley, CK; Bissessor, M; Mokany, E; Todd, AV; ...
    Date
    2016-06-06
    Source Title
    PLoS One
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Tabrizi, Sepehr; Bradshaw, Catriona; Fairley, Christopher; Wark, Suzanne; POLJAK, MARIN
    Affiliation
    University General
    Obstetrics and Gynaecology
    Melbourne School of Population and Global Health
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Tabrizi, S. N., Tan, L. Y., Walker, S., Twin, J., Poljak, M., Bradshaw, C. S., Fairley, C. K., Bissessor, M., Mokany, E., Todd, A. V. & Garland, S. M. (2016). Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology. PLOS ONE, 11 (6), https://doi.org/10.1371/journal.pone.0156740.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/259323
    DOI
    10.1371/journal.pone.0156740
    Abstract
    Mycoplasma genitalium is a cause of non-gonoccocal urethritis (NGU) in men and cervicitis and pelvic inflammatory disease in women. Recent international data also indicated that the first line treatment, 1 gram stat azithromycin therapy, for M. genitalium is becoming less effective, with the corresponding emergence of macrolide resistant strains. Increasing failure rates of azithromycin for M. genitalium has significant implications for the presumptive treatment of NGU and international clinical treatment guidelines. Assays able to predict macrolide resistance along with detection of M. genitalium will be useful to enable appropriate selection of antimicrobials to which the organism is susceptible and facilitate high levels of rapid cure. One such assay recently developed is the MG 23S assay, which employs novel PlexZyme™ and PlexPrime™ technology. It is a multiplex assay for detection of M. genitalium and 5 mutations associated with macrolide resistance. The assay was evaluated in 400 samples from 254 (186 males and 68 females) consecutively infected participants, undergoing tests of cure. Using the MG 23S assay, 83% (331/440) of samples were positive, with 56% of positives carrying a macrolide resistance mutation. Comparison of the MG 23S assay to a reference qPCR method for M. genitalium detection and high resolution melt analysis (HRMA) and sequencing for detection of macrolide resistance mutations, resulted in a sensitivity and specificity for M. genitalium detection and for macrolide resistance of 99.1/98.5% and 97.4/100%, respectively. The MG 23S assay provides a considerable advantage in clinical settings through combined diagnosis and detection of macrolide resistance.

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