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dc.contributor.authorSeth-Smith, HMB
dc.contributor.authorSanchez-Buso, L
dc.contributor.authorLivingstone, M
dc.contributor.authorSait, M
dc.contributor.authorHarris, SR
dc.contributor.authorAitchison, KD
dc.contributor.authorVretou, E
dc.contributor.authorSiarkou, VI
dc.contributor.authorLaroucau, K
dc.contributor.authorSachse, K
dc.contributor.authorLongbottom, D
dc.contributor.authorThomson, NR
dc.date.accessioned2021-02-04T01:21:57Z
dc.date.available2021-02-04T01:21:57Z
dc.date.issued2017-05-04
dc.identifierpii: 10.1186/s12864-017-3657-y
dc.identifier.citationSeth-Smith, H. M. B., Sanchez-Buso, L., Livingstone, M., Sait, M., Harris, S. R., Aitchison, K. D., Vretou, E., Siarkou, V. I., Laroucau, K., Sachse, K., Longbottom, D. & Thomson, N. R. (2017). European Chlamydia abortus livestock isolate genomes reveal unusual stability and limited diversity, reflected in geographical signatures. BMC GENOMICS, 18 (1), https://doi.org/10.1186/s12864-017-3657-y.
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/11343/259338
dc.description.abstractBACKGROUND: Chlamydia abortus (formerly Chlamydophila abortus) is an economically important livestock pathogen, causing ovine enzootic abortion (OEA), and can also cause zoonotic infections in humans affecting pregnancy outcome. Large-scale genomic studies on other chlamydial species are giving insights into the biology of these organisms but have not yet been performed on C. abortus. Our aim was to investigate a broad collection of European isolates of C. abortus, using next generation sequencing methods, looking at diversity, geographic distribution and genome dynamics. RESULTS: Whole genome sequencing was performed on our collection of 57 C. abortus isolates originating primarily from the UK, Germany, France and Greece, but also from Tunisia, Namibia and the USA. Phylogenetic analysis of a total of 64 genomes shows a deep structural division within the C. abortus species with a major clade displaying limited diversity, in addition to a branch carrying two more distantly related Greek isolates, LLG and POS. Within the major clade, seven further phylogenetic groups can be identified, demonstrating geographical associations. The number of variable nucleotide positions across the sampled isolates is significantly lower than those published for C. trachomatis and C. psittaci. No recombination was identified within C. abortus, and no plasmid was found. Analysis of pseudogenes showed lineage specific loss of some functions, notably with several Pmp and TMH/Inc proteins predicted to be inactivated in many of the isolates studied. CONCLUSIONS: The diversity within C. abortus appears to be much lower compared to other species within the genus. There are strong geographical signatures within the phylogeny, indicating clonal expansion within areas of limited livestock transport. No recombination has been identified within this species, showing that different species of Chlamydia may demonstrate different evolutionary dynamics, and that the genome of C. abortus is highly stable.
dc.languageEnglish
dc.publisherBMC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleEuropean Chlamydia abortus livestock isolate genomes reveal unusual stability and limited diversity, reflected in geographical signatures
dc.typeJournal Article
dc.identifier.doi10.1186/s12864-017-3657-y
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleBMC Genomics
melbourne.source.volume18
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1210096
melbourne.contributor.authorSait, Michelle
dc.identifier.eissn1471-2164
melbourne.accessrightsOpen Access


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