University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Dental School
  • Melbourne Dental School - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Dental School
  • Melbourne Dental School - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Cytotoxicity of Selected Nanoparticles on Human Dental Pulp Stem Cells.

    Thumbnail
    Download
    Published version (302.0Kb)

    Citations
    Altmetric
    Author
    Tabari, K; Hosseinpour, S; Parashos, P; Kardouni Khozestani, P; Rahimi, HM
    Date
    2017
    Source Title
    Iranian Endodontic Journal
    University of Melbourne Author/s
    Parashos, Peter
    Affiliation
    Melbourne Dental School
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Tabari, K., Hosseinpour, S., Parashos, P., Kardouni Khozestani, P. & Rahimi, H. M. (2017). Cytotoxicity of Selected Nanoparticles on Human Dental Pulp Stem Cells.. Iran Endod J, 12 (2), pp.137-142. https://doi.org/10.22037/iej.2017.28.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/259345
    DOI
    10.22037/iej.2017.28
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421267
    Abstract
    INTRODUCTION: Nanoparticles are being increasingly applied in dentistry due to their antimicrobial and mechanical properties. This in vitro study aimed to assess and compare the cytotoxicity of four metal oxide nanoparticles (TiO2, SiO2, ZnO, and Al2O3) on human dental pulp stem cells. METHODS AND MATERIALS: Four suspension with different concentrations (25, 50, 75, 100 µg/mL) of each nanoparticle were prepared and placed into cavities of three 96-well plates (containing 1×104 cells per well that were seeded 24 earlier). All specimens were incubated in a humidified incubator with 5% CO2 at 37°C. Mosmann's Tetrazolium Toxicity (MTT) assay was used to determine in vitro cytotoxicity of test materials on pulpal stem cells. Cell viability was determined at 24, 48, and 72 h after exposure. Data comparisons were performed using a general linear model for repeated measures and Tukey's post hoc test. The level of significance was set at 0.05. RESULTS: The tested nanoparticles showed variable levels of cytotoxicity and were dose and time dependant. The minimum cell viability was observed in ZnO followed by TiO2, SiO2 and Al2O3. CONCLUSION: The results demonstrated that cell viability and morphological modifications occurred at the concentration range of 25 to 100 µg/mL and in all nanoparticles. The higher concentration and longer duration of exposure increased cellular death. Our results highlight the need for a more discrete use of nanoparticles for biomedical applications.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52609]
    • Melbourne Dental School - Research Publications [293]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors