B cell differentiation and isotype switching is related to division cycle number.
AuthorHodgkin, PD; Lee, JH; Lyons, AB
Source TitleJournal of Experimental Medicine
PublisherRockefeller University Press
University of Melbourne Author/sHodgkin, Philip
AffiliationMedical Biology (W.E.H.I.)
Document TypeJournal Article
CitationsHodgkin, P. D., Lee, J. H. & Lyons, A. B. (1996). B cell differentiation and isotype switching is related to division cycle number.. J Exp Med, 184 (1), pp.277-281. https://doi.org/10.1084/jem.184.1.277.
Access StatusOpen Access
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192686
The mature, resting immunoglobulin (Ig) M, IgD+ B lymphocyte can be induced by T cells to proliferate, switch isotype, and differentiate into Ig-secreting or memory cells. Furthermore, B cell activation results in the de novo expression or loss of a number of cell surface molecules that function in cell recirculation or further interaction with T cells. Here, a novel fluorescent technique reveals that T-dependent B cell activation induces cell surface changes that correlate with division cycle number. Furthermore, striking stepwise changes are often centered on a single round of cell division. Particularly marked was the consistent increase in IgG1+ B cells after the second division cycle, from an initial level of < 3% IgG1+ to a plateau of approximately 40% after six cell divisions. The relationship between the percentage of IgG1+ B cells and division number was independent of time after stimulation, indicating a requirement for cell division in isotype switching. IgD expression became negative after four divisions, and a number of changes centered on the sixth division, including the loss of IgM, CD23, and B220. The techniques used here should prove useful for tracking other differentiation pathways and for future analysis of the molecular events associated with stepwise differentiation at the single cell level.
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References