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dc.contributor.authorHodgkin, PD
dc.contributor.authorLee, JH
dc.contributor.authorLyons, AB
dc.date.accessioned2021-02-04T01:27:57Z
dc.date.available2021-02-04T01:27:57Z
dc.date.issued1996-07-01
dc.identifier.citationHodgkin, P. D., Lee, J. H. & Lyons, A. B. (1996). B cell differentiation and isotype switching is related to division cycle number.. J Exp Med, 184 (1), pp.277-281. https://doi.org/10.1084/jem.184.1.277.
dc.identifier.issn0022-1007
dc.identifier.urihttp://hdl.handle.net/11343/259371
dc.description.abstractThe mature, resting immunoglobulin (Ig) M, IgD+ B lymphocyte can be induced by T cells to proliferate, switch isotype, and differentiate into Ig-secreting or memory cells. Furthermore, B cell activation results in the de novo expression or loss of a number of cell surface molecules that function in cell recirculation or further interaction with T cells. Here, a novel fluorescent technique reveals that T-dependent B cell activation induces cell surface changes that correlate with division cycle number. Furthermore, striking stepwise changes are often centered on a single round of cell division. Particularly marked was the consistent increase in IgG1+ B cells after the second division cycle, from an initial level of < 3% IgG1+ to a plateau of approximately 40% after six cell divisions. The relationship between the percentage of IgG1+ B cells and division number was independent of time after stimulation, indicating a requirement for cell division in isotype switching. IgD expression became negative after four divisions, and a number of changes centered on the sixth division, including the loss of IgM, CD23, and B220. The techniques used here should prove useful for tracking other differentiation pathways and for future analysis of the molecular events associated with stepwise differentiation at the single cell level.
dc.languageeng
dc.publisherRockefeller University Press
dc.titleB cell differentiation and isotype switching is related to division cycle number.
dc.typeJournal Article
dc.identifier.doi10.1084/jem.184.1.277
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleJournal of Experimental Medicine
melbourne.source.volume184
melbourne.source.issue1
melbourne.source.pages277-281
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1208885
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192686
melbourne.contributor.authorHodgkin, Philip
dc.identifier.eissn1540-9538
melbourne.accessrightsOpen Access


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