Show simple item record

dc.contributor.authorHolz, LE
dc.contributor.authorJakobsen, KP
dc.contributor.authorVan Snick, J
dc.contributor.authorCormont, F
dc.contributor.authorSewell, WA
dc.date.accessioned2021-02-04T01:29:12Z
dc.date.available2021-02-04T01:29:12Z
dc.date.issued2005
dc.identifierpii: 1476-9255-2-3
dc.identifier.citationHolz, L. E., Jakobsen, K. P., Van Snick, J., Cormont, F. & Sewell, W. A. (2005). Dexamethasone inhibits IL-9 production by human T cells.. J Inflamm (Lond), 2 (1), pp.3-. https://doi.org/10.1186/1476-9255-2-3.
dc.identifier.issn1476-9255
dc.identifier.urihttp://hdl.handle.net/11343/259378
dc.description.abstractBACKGROUND: Interleukin 9 (IL-9) is produced by activated CD4+ T cells. Its effects include stimulation of mucus production, enhanced mast cell proliferation, enhanced eosinophil function, and IgE production. These effects are consistent with a role in allergic diseases. Glucocorticoids have potent anti-inflammatory effects, including suppression of cytokine synthesis, and are widely used in the treatment of allergic conditions. METHODS: We examined the effect of the glucocorticoid dexamethasone (Dex) on IL-9 mRNA expression and protein secretion with real-time RT-PCR and ELISA. Peripheral blood mononuclear cells (PBMC) were prepared from human volunteers and activated with OKT3. CD4+ T cells were purified from PBMC and activated with OKT3 plus PMA. RESULTS: IL-9 mRNA abundance and protein secretion were both markedly reduced following treatment of activated PBMC with Dex. mRNA levels were reduced to 0.7% of control values and protein secretion was reduced to 2.8% of controls. In CD4+ T cells, Dex reduced protein secretion to a similar extent. The IC50 value of Dex on mRNA expression was 4 nM. CONCLUSION: These results indicate that IL-9 production is very markedly inhibited by Dex. The findings raise the possibility that the beneficial effects of glucocorticoids in the treatment of allergic diseases are in part mediated by inhibition of IL-9 production.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.titleDexamethasone inhibits IL-9 production by human T cells.
dc.typeJournal Article
dc.identifier.doi10.1186/1476-9255-2-3
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleJournal of Inflammation
melbourne.source.volume2
melbourne.source.issue1
melbourne.source.pages3-
dc.rights.licenseCC BY
melbourne.elementsid1209771
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1112611
melbourne.contributor.authorHolz, Lauren
dc.identifier.eissn1476-9255
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record