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dc.contributor.authorJacob, L
dc.contributor.authorGagnon-Bartsch, JA
dc.contributor.authorSpeed, TP
dc.date.accessioned2021-02-04T01:35:59Z
dc.date.available2021-02-04T01:35:59Z
dc.date.issued2016-01-01
dc.identifierpii: kxv026
dc.identifier.citationJacob, L., Gagnon-Bartsch, J. A. & Speed, T. P. (2016). Correcting gene expression data when neither the unwanted variation nor the factor of interest are observed. BIOSTATISTICS, 17 (1), pp.16-28. https://doi.org/10.1093/biostatistics/kxv026.
dc.identifier.issn1465-4644
dc.identifier.urihttp://hdl.handle.net/11343/259415
dc.description.abstractWhen dealing with large scale gene expression studies, observations are commonly contaminated by sources of unwanted variation such as platforms or batches. Not taking this unwanted variation into account when analyzing the data can lead to spurious associations and to missing important signals. When the analysis is unsupervised, e.g. when the goal is to cluster the samples or to build a corrected version of the dataset--as opposed to the study of an observed factor of interest--taking unwanted variation into account can become a difficult task. The factors driving unwanted variation may be correlated with the unobserved factor of interest, so that correcting for the former can remove the latter if not done carefully. We show how negative control genes and replicate samples can be used to estimate unwanted variation in gene expression, and discuss how this information can be used to correct the expression data. The proposed methods are then evaluated on synthetic data and three gene expression datasets. They generally manage to remove unwanted variation without losing the signal of interest and compare favorably to state-of-the-art corrections. All proposed methods are implemented in the bioconductor package RUVnormalize.
dc.languageEnglish
dc.publisherOXFORD UNIV PRESS
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleCorrecting gene expression data when neither the unwanted variation nor the factor of interest are observed
dc.typeJournal Article
dc.identifier.doi10.1093/biostatistics/kxv026
melbourne.affiliation.departmentSchool of Mathematics and Statistics
melbourne.affiliation.facultyScience
melbourne.source.titleBiostatistics
melbourne.source.volume17
melbourne.source.issue1
melbourne.source.pages16-28
dc.rights.licenseCC BY
melbourne.elementsid1212069
melbourne.contributor.authorSpeed, Terence
dc.identifier.eissn1468-4357
melbourne.accessrightsOpen Access


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