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dc.contributor.authorYeung, ATY
dc.contributor.authorHale, C
dc.contributor.authorXia, J
dc.contributor.authorTate, PH
dc.contributor.authorGoulding, D
dc.contributor.authorKeane, JA
dc.contributor.authorMukhopadhyay, S
dc.contributor.authorForrester, L
dc.contributor.authorBillker, O
dc.contributor.authorSkarnes, WC
dc.contributor.authorHancock, REW
dc.contributor.authorDougan, G
dc.date.accessioned2021-02-04T01:50:56Z
dc.date.available2021-02-04T01:50:56Z
dc.date.issued2015-03-10
dc.identifierpii: srep08908
dc.identifier.citationYeung, A. T. Y., Hale, C., Xia, J., Tate, P. H., Goulding, D., Keane, J. A., Mukhopadhyay, S., Forrester, L., Billker, O., Skarnes, W. C., Hancock, R. E. W. & Dougan, G. (2015). Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function.. Sci Rep, 5 (1), pp.8908-. https://doi.org/10.1038/srep08908.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/259495
dc.description.abstractThe ability to differentiate genetically modified mouse embryonic stem (ES) cells into functional macrophages provides a potentially attractive resource to study host-pathogen interactions without the need for animal experimentation. This is particularly useful in instances where the gene of interest is essential and a knockout mouse is not available. Here we differentiated mouse ES cells into macrophages in vitro and showed, through a combination of flow cytometry, microscopic imaging, and RNA-Seq, that ES cell-derived macrophages responded to S. Typhimurium, in a comparable manner to mouse bone marrow derived macrophages. We constructed a homozygous mutant mouse ES cell line in the Traf2 gene that is known to play a role in tumour necrosis factor-α signalling but has not been studied for its role in infections or response to Toll-like receptor agonists. Interestingly, traf2-deficient macrophages produced reduced levels of inflammatory cytokines in response to lipopolysaccharide (LPS) or flagellin stimulation and exhibited increased susceptibility to S. Typhimurium infection.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.titleConditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function.
dc.typeJournal Article
dc.identifier.doi10.1038/srep08908
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleScientific Reports
melbourne.source.volume5
melbourne.source.issue1
melbourne.source.pages8908-
dc.rights.licenseCC BY
melbourne.elementsid1080589
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354151
melbourne.contributor.authorDougan, Gordon
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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