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    Functional genomics reveals that Clostridium difficile Spo0A coordinates sporulation, virulence and metabolism

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    52
    Author
    Pettit, LJ; Browne, HP; Yu, L; Smits, WK; Fagan, RP; Barquist, L; Martin, MJ; Goulding, D; Duncan, SH; Flint, HJ; ...
    Date
    2014-02-25
    Source Title
    BMC Genomics
    Publisher
    BMC
    University of Melbourne Author/s
    Dougan, Gordon
    Affiliation
    Microbiology and Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Pettit, L. J., Browne, H. P., Yu, L., Smits, W. K., Fagan, R. P., Barquist, L., Martin, M. J., Goulding, D., Duncan, S. H., Flint, H. J., Dougan, G., Choudhary, J. S. & Lawley, T. D. (2014). Functional genomics reveals that Clostridium difficile Spo0A coordinates sporulation, virulence and metabolism. BMC GENOMICS, 15 (1), https://doi.org/10.1186/1471-2164-15-160.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/259498
    DOI
    10.1186/1471-2164-15-160
    Abstract
    BACKGROUND: Clostridium difficile is an anaerobic, Gram-positive bacterium that can reside as a commensal within the intestinal microbiota of healthy individuals or cause life-threatening antibiotic-associated diarrhea in immunocompromised hosts. C. difficile can also form highly resistant spores that are excreted facilitating host-to-host transmission. The C. difficile spo0A gene encodes a highly conserved transcriptional regulator of sporulation that is required for relapsing disease and transmission in mice. RESULTS: Here we describe a genome-wide approach using a combined transcriptomic and proteomic analysis to identify Spo0A regulated genes. Our results validate Spo0A as a positive regulator of putative and novel sporulation genes as well as components of the mature spore proteome. We also show that Spo0A regulates a number of virulence-associated factors such as flagella and metabolic pathways including glucose fermentation leading to butyrate production. CONCLUSIONS: The C. difficile spo0A gene is a global transcriptional regulator that controls diverse sporulation, virulence and metabolic phenotypes coordinating pathogen adaptation to a wide range of host interactions. Additionally, the rich breadth of functional data allowed us to significantly update the annotation of the C. difficile 630 reference genome which will facilitate basic and applied research on this emerging pathogen.

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