Targeting of a Transporter to the Outer Apicoplast Membrane in the Human Malaria Parasite Plasmodium falciparum

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Lim, L; Sayers, CP; Goodman, CD; McFadden, GIDate
2016-07-21Source Title
PLoS OnePublisher
PUBLIC LIBRARY SCIENCEUniversity of Melbourne Author/s
Goodman, Christopher; Sayers, Claire; McFadden, Geoffrey; LIM, LITINGAffiliation
School of BioSciencesMetadata
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Journal ArticleCitations
Lim, L., Sayers, C. P., Goodman, C. D. & McFadden, G. I. (2016). Targeting of a Transporter to the Outer Apicoplast Membrane in the Human Malaria Parasite Plasmodium falciparum. PLOS ONE, 11 (7), https://doi.org/10.1371/journal.pone.0159603.Access Status
Open AccessARC Grant code
ARC/DP130103236Abstract
Apicoplasts are vestigial plastids in apicomplexan parasites like Plasmodium, the causative agent of malaria. Apicomplexan parasites are dependant on their apicoplasts for synthesis of various molecules that they are unable to scavenge in sufficient quantity from their host, which makes apicoplasts attractive drug targets. Proteins known as plastid phosphate translocators (pPTs) are embedded in the outer apicoplast membrane and are responsible for the import of carbon, energy and reducing power to drive anabolic synthesis in the organelle. We investigated how a pPT is targeted into the outer apicoplast membrane of the human malaria parasite P. falciparum. We showed that a transmembrane domain is likely to act as a recessed signal anchor to direct the protein into the endomembrane system, and that a tyrosine in the cytosolic N-terminus of the protein is essential for targeting, but one or more, as yet unidentified, factors are also essential to direct the protein into the outer apicoplast membrane.
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