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dc.contributor.authorColussi, PA
dc.contributor.authorQuinn, LM
dc.contributor.authorHuang, DCS
dc.contributor.authorCoombe, M
dc.contributor.authorRead, SH
dc.contributor.authorRichardson, H
dc.contributor.authorKumar, S
dc.date.accessioned2021-02-04T01:55:02Z
dc.date.available2021-02-04T01:55:02Z
dc.date.issued2000-02-21
dc.identifier.citationColussi, P. A., Quinn, L. M., Huang, D. C. S., Coombe, M., Read, S. H., Richardson, H. & Kumar, S. (2000). Debcl, a proapoptotic Bcl-2 homologue, is a component of the Drosophila melanogaster cell death machinery. JOURNAL OF CELL BIOLOGY, 148 (4), pp.703-714. https://doi.org/10.1083/jcb.148.4.703.
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/11343/259517
dc.description.abstractBcl-2 family of proteins are key regulators of apoptosis. Both proapoptotic and antiapoptotic members of this family are found in mammalian cells, but no such proteins have been described in insects. Here, we report the identification and characterization of Debcl, the first Bcl-2 homologue in Drosophila melanogaster. Structurally, Debcl is similar to Bax-like proapoptotic Bcl-2 family members. Ectopic expression of Debcl in cultured cells and in transgenic flies causes apoptosis, which is inhibited by coexpression of the baculovirus caspase inhibitor P35, indicating that Debcl is a proapoptotic protein that functions in a caspase-dependent manner. debcl expression correlates with developmental cell death in specific Drosophila tissues. We also show that debcl genetically interacts with diap1 and dark, and that debcl-mediated apoptosis is not affected by gene dosage of rpr, hid, and grim. Biochemically, Debcl can interact with several mammalian and viral prosurvival Bcl-2 family members, but not with the proapoptotic members, suggesting that it may regulate apoptosis by antagonizing prosurvival Bcl-2 proteins. RNA interference studies indicate that Debcl is required for developmental apoptosis in Drosophila embryos. These results suggest that the main components of the mammalian apoptosis machinery are conserved in insects.
dc.languageEnglish
dc.publisherROCKEFELLER UNIV PRESS
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.titleDebcl, a proapoptotic Bcl-2 homologue, is a component of the Drosophila melanogaster cell death machinery
dc.typeJournal Article
dc.identifier.doi10.1083/jcb.148.4.703
melbourne.affiliation.departmentAnatomy and Neuroscience
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleThe Journal of Cell Biology
melbourne.source.volume148
melbourne.source.issue4
melbourne.source.pages703-714
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1083805
melbourne.contributor.authorRichardson, Helena
melbourne.contributor.authorQuinn, Leonie
melbourne.contributor.authorHuang, David
dc.identifier.eissn1540-8140
melbourne.accessrightsOpen Access


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