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    Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS

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    5
    Author
    Livingstone, M; Wheelhouse, N; Ensor, H; Rocchi, M; Maley, S; Aitchison, K; Wattegedera, S; Wilson, K; Sait, M; Siarkou, V; ...
    Date
    2017-05-11
    Source Title
    PLoS One
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Sait, Michelle
    Affiliation
    Microbiology and Immunology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Livingstone, M., Wheelhouse, N., Ensor, H., Rocchi, M., Maley, S., Aitchison, K., Wattegedera, S., Wilson, K., Sait, M., Siarkou, V., Vretou, E., Entrican, G., Dagleish, M. & Longbottom, D. (2017). Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLOS ONE, 12 (5), https://doi.org/10.1371/journal.pone.0177653.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/259567
    DOI
    10.1371/journal.pone.0177653
    Abstract
    This study investigated the pathogenesis of two variant strains (LLG and POS) of Chlamydia abortus, in comparison to a typical wild-type strain (S26/3) which is known to be responsible for late term abortion in small ruminants. Challenge with the three strains at mid-gestation resulted in similar pregnancy outcomes, with abortion occurring in approximately 50-60% of ewes with the mean gestational lengths also being similar. However, differences were observed in the severity of placental pathology, with infection appearing milder for strain LLG, which was reflected in the lower number of organisms shed in vaginal swabs post-partum and less gross pathology and organisms present in placental smears. Results for strain POS were somewhat different than LLG with a more focal restriction of infection observed. Post-abortion antibody responses revealed prominent differences in seropositivity to the major outer membrane protein (MOMP) present in elementary body (EB) preparations under denaturing conditions, most notably with anti-LLG and anti-POS convalescent sera where there was no or reduced detection of MOMP present in EBs derived from the three strains. These results and additional analysis of whole EB and chlamydial outer membrane complex preparations suggest that there are conformational differences in MOMP for the three strains. Overall, the results suggest that gross placental pathology and clinical outcome is not indicative of bacterial colonization and the severity of infection. The results also highlight potential conformational differences in MOMP epitopes that perhaps impact on disease diagnosis and the development of new vaccines.

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