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dc.contributor.authorDeng, Y-M
dc.contributor.authorCaldwell, N
dc.contributor.authorBarr, IG
dc.date.accessioned2021-02-04T02:10:54Z
dc.date.available2021-02-04T02:10:54Z
dc.date.issued2011-08-19
dc.identifierpii: PONE-D-11-10048
dc.identifier.citationDeng, Y. -M., Caldwell, N. & Barr, I. G. (2011). Rapid Detection and Subtyping of Human Influenza A Viruses and Reassortants by Pyrosequencing. PLOS ONE, 6 (8), https://doi.org/10.1371/journal.pone.0023400.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/259598
dc.description.abstractBACKGROUND: Given the continuing co-circulation of the 2009 H1N1 pandemic influenza A viruses with seasonal H3N2 viruses, rapid and reliable detection of newly emerging influenza reassortant viruses is important to enhance our influenza surveillance. METHODOLOGY/PRINCIPAL FINDINGS: A novel pyrosequencing assay was developed for the rapid identification and subtyping of potential human influenza A virus reassortants based on all eight gene segments of the virus. Except for HA and NA genes, one universal set of primers was used to amplify and subtype each of the six internal genes. With this method, all eight gene segments of 57 laboratory isolates and 17 original specimens of seasonal H1N1, H3N2 and 2009 H1N1 pandemic viruses were correctly matched with their corresponding subtypes. In addition, this method was shown to be capable of detecting reassortant viruses by correctly identifying the source of all 8 gene segments from three vaccine production reassortant viruses and three H1N2 viruses. CONCLUSIONS/SIGNIFICANCE: In summary, this pyrosequencing assay is a sensitive and specific procedure for screening large numbers of viruses for reassortment events amongst the commonly circulating human influenza A viruses, which is more rapid and cheaper than using conventional sequencing approaches.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleRapid Detection and Subtyping of Human Influenza A Viruses and Reassortants by Pyrosequencing
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0023400
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titlePLoS One
melbourne.source.volume6
melbourne.source.issue8
dc.rights.licenseCC BY
melbourne.elementsid1219010
melbourne.contributor.authorBarr, Ian
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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