Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome
AuthorLesche, D; Geyer, R; Lienhard, D; Nakas, CT; Diserens, G; Vermathen, P; Leichtle, AB
University of Melbourne Author/sLesche, Dorothea
AffiliationSir Peter MacCallum Department of Oncology
Document TypeJournal Article
CitationsLesche, D., Geyer, R., Lienhard, D., Nakas, C. T., Diserens, G., Vermathen, P. & Leichtle, A. B. (2016). Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome. METABOLOMICS, 12 (10), https://doi.org/10.1007/s11306-016-1109-3.
Access StatusOpen Access
BACKGROUND: Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs. AIM: Determine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses. METHODS: Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements. RESULTS: Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols. CONCLUSION: Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.
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